RT Journal Article SR Electronic(1) A1 Schellenberg, Johannes A1 Busse, Hans-Jürgen A1 Hardt, Martin A1 Schubert, Patrick A1 Wilke, Thomas A1 Kämpfer, Peter A1 Glaeser, Stefanie P.YR 2017 T1 Winogradskyella haliclonae sp. nov., isolated from a marine sponge of the genus Haliclona JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 12 SP 4902 OP 4910 DO https://doi.org/10.1099/ijsem.0.002192 PB Microbiology Society, SN 1466-5034, AB A yellow-pigmented, Gram-stain-negative, motile and rod-shaped bacterium, strain M1A16T, was isolated from the internal tissue of a sponge of the genus Haliclona, which was long-term cultured in the CEMarin aquaria system at Justus Liebig University of Giessen. The strain grew well at 20–32 °C (optimum 25 °C), in the presence of 0–6 % NaCl (optimum 3 %), and at pH 5.5–9.0 (optimum pH 7.0–8.0). Phylogenetic analysis based on its 16S rRNA gene sequence placed the strain within the monophyletic cluster of the genus Winogradskyella with highest sequence similarity to Winogradskyella jejuensis CP32T (98.3 % 16S rRNA gene sequence similarity). Sequence similarities to all other type strains were 98.0 % or less. DNA–DNA hybridization of strain M1A16T with W. jejuensis CP32T resulted in hybridization values of 44.1 % (reciprocal 68.1 %). Major cellular fatty acids of strain M1A16T were iso-C15 : 1 G (18.1 %), iso-C15 : 0 (13.7 %), C16 : 1ω7c (12.9 %), iso-C17 : 0 3-OH (10.6 %) and iso-C16 : 0 3-OH (10.2 %). The overall polyamine content was very low with major components being cadaverine, spermidine and sym-homospermidine. The major quinone was menaquinone MK-6. The polar lipid profile contained predominantly phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids devoid of a detectable functional group. The genomic DNA G+C content was 32.7 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic analyses, strain M1A16T represents a novel species of the genus Winogradskyella , for which the name Winogradskyella haliclonae sp. nov. is proposed. The type strain is M1A16T (=DSM 103138T=CCM 8681T=LMG 29588T=CIP 111091T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002192