1887

Abstract

A Gram-negative, aerobic, non-motile, long rods or coccoid without flagellum strain, designated THG-YS3.2.7, was isolated from therhizosphere soil of a Mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10–40 °C (optimum 28–37 °C), at pH 6–8 (optimum 7) and at 0–5 % NaCl (optimum 1 %). The predominant ubiquinone was ubiquinone 8 (Q-8). The major cellular fatty acids were C, C 3OH, C, C, C cyclo, C, C 6 (6,9,12), summed feature 3 (Cω7 and/or Cω6) and summed feature 8 (C 7 and/or C 6). The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC). The DNA G+C content of strain THG-YS3.2.7 was 69.4 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.2.7 were identified as DSM 14656 (97.92 %), DSM 14655 (97.90 %), KACC 17567 (97.04 %). DNA–DNA hybridization values between strain THG-YS3.2.7 and DSM 14656, DSM 14655, KACC 17567 were 32.5±1.5, 43.1±1.1, 42.8±1.1 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-YS3.2.7 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is THG-YS3.2.7 (=KCTC 52083=CCTCC AB 2015357).

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2017-10-01
2019-12-12
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