@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002190, author = "Hyeon, Jong Woo and Kim, Kyung Hyun and Chun, Byung Hee and Jeon, Che Ok", title = "Pontibacterium granulatum gen. nov., sp. nov., isolated from a tidal flat", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "10", pages = "3784-3790", doi = "https://doi.org/10.1099/ijsem.0.002190", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002190", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Pontibacterium granulatum", keywords = "tidal fla", keywords = "PHA", keywords = "new taxa", keywords = "Oceanospirillaceae", abstract = "A Gram-stain-negative, strictly aerobic, moderately halophilic bacterium, designated A-1T, was isolated from a tidal flat of the Taean coast in South Korea. Cells were motile rods with a single flagellum showing oxidase-negative and catalase-positive activities and contained poly-β-hydroxyalkanoic acid granules. Growth of strain A-1T was observed at 20–40 °C (optimum, 30 °C), pH 6.0–10.5 (optimum, pH 7.0) and in the presence of 1.0–6.0 % (w/v) NaCl (optimum, 2.0 %). Strain A-1T contained C16 : 0, summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (comprising C18 : 1 ω7c and/or C18 : 1 ω6c) as the major fatty acids. The major polar lipids of strain A-1T were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The isoprenoid quinones detected were ubiquinone-7 and ubiquinone-8. The G+C content of the genomic DNA was 51.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain A-1T formed a distinct phylogenetic lineage from other genera within the family Oceanospirillaceae . Strain A-1T shared low 16S rRNA gene sequence similarities with other taxa (≤94.9 %). On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that strain A-1T represents a novel genus and species of the family Oceanospirillaceae , for which the name Pontibacterium granulatum gen. nov., sp. nov. is proposed. The type strain is A-1T (=KACC 18119T=JCM 30136T).", }