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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 9

Pathogenic, phenotypic and molecular characterisation of sp. nov. and sp. nov., new species of associated with watercress production in Florida Free

Abstract

We describe two new species of the genus , represented by yellow mucoid bacterial strains isolated from diseased leaves of watercress () produced in Florida, USA. One strain was pathogenic on watercress, but not in other species including a range of brassicas; other strains were not pathogenic in any of the tested plants. Data from Biolog carbon source utilization tests and nucleotide sequence data from 16S and loci suggested that both pathogenic and non-pathogenic strains were related to, yet distinct from, previously described species. Multilocus sequence analysis and whole genome-wide comparisons of the average nucleotide identity (ANI) of genomes of two strains from watercress showed that these are distinct and share less than 95 % ANI with all other known species; the non-pathogenic strain WHRI 8848 is close to (ANI of 93.72 %) whilst the pathogenic strain WHRI 8853 is close to a large clade of species that includes (ANI ≤90.25 %). Based on these results, we propose that both strains represent new species named sp. nov. (type strain WHRI 8848=ATCC TSD-60=ICMP 21312=LMG 29665=NCPPB 4601) and sp. nov. (type strain WHRI 8853=ATCC TSD-61=ICMP 21313=LMG 29666=NCPPB 4600), respectively. The presence of non-pathogenic strains in watercress and their interaction with pathogenic strains needs to be further investigated. Although the importance of the new pathogenic species is yet to be determined, the bacterial disease that it causes constitutes a threat to watercress production and its distribution should be monitored.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): black rot , Brassicaceae , new species , pathogenicity , watercress and Xanthomonas
© 2017 IUMS
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2017-09-01
2024-04-19
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Pathogenic, phenotypic and molecular characterisation of Xanthomonas nasturtii sp. nov. and Xanthomonas floridensis sp. nov., new species of Xanthomonas associated with watercress production in Florida
Int J Syst Evol Microbiol 67, 3645 (2017); https://doi.org/10.1099/ijsem.0.002189
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