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Volume 67, Issue 10

gen. nov., sp. nov., a propanil-degrading bacterium Free

Abstract

Strain EPL6, a Gram-negative, motile, short rod was isolated from a propanil and 3,4-dichloroaniline enrichment culture produced from rice paddy soil. Based on the analyses of the 16S rRNA gene sequence, strain EPL6 was observed to be a member of the family , sharing the highest pairwise identity with type strains of the species K601 (96.8 %) and CY1 (96.8 %). Strain EPL6 was able to grow in a temperature range of 15–37 °C, pH 6–9 and in the presence of up to 4 % (w/v) NaCl and tested positive for catalase and oxidase reactions. The major respiratory quinone was Q8. The genomic DNA had a G+C content of 69.4±0.9 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and the major fatty acid methyl esters comprised C, Cω7 and summed feature 3 (Cω7/iso-C 2-OH). Comparison of the genome sequence of strain EPL6 and of its closest neighbours, CY1 and K601, yielded values of ANI ≤84.1 % and of AAI ≤80.3 %. Therefore, the genetic, phylogenetic, phenotypic and chemotaxonomic characteristics support the classification of this organism into a new taxon. Considering the genetic divergence of strain EPL6 from the type strains of the closest species, which belong to distinct genera, we propose a new genus within the family , named gen. nov., sp. nov., represented by the isolate EPL6 as the type strain of the species (=LMG 28427=CECT 8927).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Comamonadaceae , herbicide degrader , new genus and rice field soil
© 2017 IUMS
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2017-10-01
2024-04-19
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Oryzisolibacter propanilivorax gen. nov., sp. nov., a propanil-degrading bacterium
Int J Syst Evol Microbiol 67, 3752 (2017); https://doi.org/10.1099/ijsem.0.002184
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