1887

Abstract

Strain EPL6, a Gram-negative, motile, short rod was isolated from a propanil and 3,4-dichloroaniline enrichment culture produced from rice paddy soil. Based on the analyses of the 16S rRNA gene sequence, strain EPL6 was observed to be a member of the family Comamonadaceae , sharing the highest pairwise identity with type strains of the species Alicycliphilus denitrificans K601 (96.8 %) and Melaminivora alkalimesophila CY1 (96.8 %). Strain EPL6 was able to grow in a temperature range of 15–37 °C, pH 6–9 and in the presence of up to 4 % (w/v) NaCl and tested positive for catalase and oxidase reactions. The major respiratory quinone was Q8. The genomic DNA had a G+C content of 69.4±0.9 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and the major fatty acid methyl esters comprised C16 : 0, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c/iso-C15 : 0 2-OH). Comparison of the genome sequence of strain EPL6 and of its closest neighbours, Melaminivora alkalimesophila CY1 and Alicycliphilus denitrificans K601, yielded values of ANI ≤84.1 % and of AAI ≤80.3 %. Therefore, the genetic, phylogenetic, phenotypic and chemotaxonomic characteristics support the classification of this organism into a new taxon. Considering the genetic divergence of strain EPL6 from the type strains of the closest species, which belong to distinct genera, we propose a new genus within the family Comamonadaceae , named Oryzisolibacter propanilivorax gen. nov., sp. nov., represented by the isolate EPL6 as the type strain of the species (=LMG 28427=CECT 8927).

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2017-09-12
2019-10-15
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