1887

Abstract

Two Gram-stain-negative, facultatively anaerobic, motile bacterial strains isolated from symptomatic bark of canker in China were investigated using a polyphasic approach, including 16S rRNA gene sequencing, multilocus sequence analysis, and biochemical and physiological assays. 16S rRNA gene sequencing indicated that these strains belonged to the genus , family , and had the highest sequence similarity with CFCC 11963 (98 %). DNA–DNA hybridization experiments revealed DNA–DNA relatedness values of 72.1–78.2 % between the new isolates and strains of , revealing that these strains belonged to the same species. The 16S rRNA gene sequencing and multilocus sequence analysis suggested that the two novel strains and those of are phylogenetically closely related but form two clearly separated subgroups. Based on the data, the two novel isolates represent a subspecies of for which the name subsp. subsp. nov. is proposed with D8-10-4-5 (=CFCC 11935=KCTC 42841) as the type strain, with the automatic creation of subsp. subsp. nov. (type strain D9-5=CFCC 11963=KCTC 42088).

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2017-09-01
2020-12-03
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