1887

Abstract

A novel bacterial strain, designated HSG9, was isolated from aisolated from a rotten tropical mangrove root. Cells of strain HSG9 were aerobic, Gram-stain-negative, yellow, oxidase-negative and catalase-positive. Growth was observed in 0.5–9 % sea salt (optimum 3 %, w/v), at 10–42 °C (optimum 25–35 °C) and at pH 6.0–8.0 (optimum 7.0–8.0). Gelatin, esterase and Tweens 20, 40, 60 and 80 were hydrolysed, but starch, protein, cellulose and casein were not. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain HSG9 formed an independent lineage related to the family Flavobacteriaceae . The dominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G. The respiratory quinone was identified as MK-6 and the polar lipids were phosphatidylethanolamine, three unidentified phospholipids and an unidentified lipid. The DNA G+C content was 37.1 mol%. The combined genotypic and phenotypic data indicated that strain HSG9 represents a novel species of a new genus within the family Flavobacteriaceae , for which the name Croceivirgaradicis gen. nov., sp. nov. is proposed. The type strain is HSG9 (=MCCC 1A06690=KCTC 52589).

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2017-09-12
2019-10-20
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References

  1. Lee HS, Kwon KK, Yang SH, Bae SS, Park CH et al. Description of Croceitalea gen. nov. in the family Flavobacteriaceae with two species, Croceitalea eckloniae sp. nov. and Croceitalea dokdonensis sp. nov., isolated from the rhizosphere of the marine alga Ecklonia kurome. Int J Syst Evol Microbiol 2008;58:2505–2510 [CrossRef][PubMed]
    [Google Scholar]
  2. Liu X, Lai Q, Du Y, Li G, Sun F et al. Tamlana nanhaiensis sp. nov., isolated from surface seawater collected from the South China Sea. Antonie van Leeuwenhoek 2015;107:1189–1196 [CrossRef][PubMed]
    [Google Scholar]
  3. Oh C, Heo SJ, de Zoysa M, Affan A, Jung WK et al. Whole-genome sequence of the xylanase-producing Mesoflavibacter zeaxanthinifaciens strain S86. J Bacteriol 2011;193:5557 [CrossRef][PubMed]
    [Google Scholar]
  4. Delong EF. Archaea in coastal marine environments. Proc Natl Acad Sci USA 1992;89:5685–5689 [CrossRef][PubMed]
    [Google Scholar]
  5. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  6. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  7. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  8. Rzhetsky A, Nei M. Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 1992;35:367–375 [CrossRef][PubMed]
    [Google Scholar]
  9. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  10. Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W et al. Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 2014;12:635–645 [CrossRef][PubMed]
    [Google Scholar]
  11. Bernardet JF, Nakagawa Y, Holmes B.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002;52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  12. Lu Y, Liu X. Determination of biochemical characteristics. In Dong XZ, Cai MY. (editors) Determinative Manual for Routine Bacteriology Beijing, FL: Scientific Press; 2001; pp.370–398
    [Google Scholar]
  13. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI; 1990
    [Google Scholar]
  14. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  15. Collins MD. Isoprenoid quinone analyses in bacterial classification and identification. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London, FL: Academic Press; 1985; pp.267–287
    [Google Scholar]
  16. Zhang G, Yang Y, Yin X, Wang S. Paracoccus pacificus sp. nov., isolated from the Western Pacific Ocean. Antonie van Leeuwenhoek 2014;106:725–731 [CrossRef][PubMed]
    [Google Scholar]
  17. Park S, Yoshizawa S, Muramatsu Y, Nakagawa Y, Yokota A et al. Aureicoccus marinus gen. nov., sp. nov., a member of the family Flavobacteriaceae, isolated from seawater. Int J Syst Evol Microbiol 2013;63:1885–1890 [CrossRef][PubMed]
    [Google Scholar]
  18. Yoon JH, Kang SJ, Jung YT, Oh TK. Muricauda lutimaris sp. nov., isolated from a tidal flat of the Yellow Sea. Int J Syst Evol Microbiol 2008;58:1603–1607 [CrossRef][PubMed]
    [Google Scholar]
  19. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001;51:1997–2006 [CrossRef][PubMed]
    [Google Scholar]
  20. Montero-Calasanz MC, Göker M, Rohde M, Spröer C, Schumann P et al. Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii, Chryseobacterium indologenes, Chryseobacterium wanjuense and Chryseobacterium gregarium. Int J Syst Evol Microbiol 2013;63:4386–4395 [CrossRef][PubMed]
    [Google Scholar]
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