1887

Abstract

Two strains of aerobic, budding, pink-pigmented bacteria, P12 and P515, were isolated from a lichen-dominated peatland and a forested tundra soil of north-western Siberia, respectively. Cells of these isolates were represented by non-motile spheres that occurred singly or were arranged in short chains and aggregates. While growing on solid media, cells of strains P12 and P515 attached to the surface by means of holdfast-like appendages. These isolates were mildly acidophilic (optimum growth at pH 5.5–6.0), psychrotolerant bacteria, which displayed tolerance of low temperatures (4–15 °C), grew optimally at 15–22 °C and did not grow at temperatures above 28 °C. The preferred growth substrates were sugars and some heteropolysaccharides. The major fatty acids were C18 : 1ω9c, C16 : 0 and C14 : 0. Trimethylornithine lipid was the major polar lipid. The only quinone was MK-6, and the G+C content of the DNA was 61.2–62.2 mol%. Strains P12 and P515 possessed identical 16S rRNA gene sequences, which affiliated them with the family Isosphaeraceae , order Planctomycetales , and these displayed the highest similarity (93–94 %) to 16S rRNA gene sequences from members of the genus Singulisphaera . However, the signature fatty acid of species of the genus Singulisphaera , i.e. C18 : 2 ω6c,12c, was absent in cells of strains P12 and P515. They also differed from members of the genus Singulisphaera by substrate utilization pattern and a number of physiological characteristics. Based on these data, the novel isolates should be considered as representing a novel genus and species of planctomycetes, for which the name Tundrisphaera lichenicola gen. nov., sp. nov, is proposed. The type strain is P12 (=LMG 29571=VKM B-3044).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002172
2017-08-22
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/9/3583.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002172&mimeType=html&fmt=ahah

References

  1. Kulichevskaya IS, Ivanova AA, Suzina NE, Rijpstra WI, Sinninghe Damsté JS et al. Paludisphaera borealis gen. nov., sp. nov., a hydrolytic planctomycete from northern wetlands, and the proposal of Isosphaeraceae fam. nov. Int J Syst Evol Microbiol 2015;66:837–844 [CrossRef][PubMed]
    [Google Scholar]
  2. Giovannoni SJ, Schabtach E, Castenholz RW. Isosphaera pallida, gen. and comb. nov., a gliding, budding eubacterium from hot springs. Arch Microbiol 1987;147:276–284 [CrossRef]
    [Google Scholar]
  3. Kulichevskaya IS, Ivanova AO, Baulina OI, Bodelier PL, Damsté JS et al. Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands. Int J Syst Evol Microbiol 2008;58:1186–1193 [CrossRef][PubMed]
    [Google Scholar]
  4. Kulichevskaya IS, Detkova EN, Bodelier PL, Rijpstra WI, Damsté JS et al. Singulisphaera rosea sp. nov., a planctomycete from acidic Sphagnum peat, and emended description of the genus Singulisphaera. Int J Syst Evol Microbiol 2012;62:118–123 [CrossRef][PubMed]
    [Google Scholar]
  5. Bondoso J, Albuquerque L, Nobre MF, Lobo-da-Cunha A, da Costa MS et al. Aquisphaera giovannonii gen. nov., sp. nov., a planctomycete isolated from a freshwater aquarium. Int J Syst Evol Microbiol 2011;61:2844–2850 [CrossRef][PubMed]
    [Google Scholar]
  6. Kulichevskaia IS, Belova SE, Kevbrin VV, Dedysh SN, Zavarzin GA. [Analysis of the bacterial community developing in the course of Sphagnum moss decomposition]. Mikrobiologiia 2007;76:621–629 [CrossRef][PubMed]
    [Google Scholar]
  7. Serkebaeva YM, Kim Y, Liesack W, Dedysh SN. Pyrosequencing-based assessment of the bacteria diversity in surface and subsurface peat layers of a northern wetland, with focus on poorly studied phyla and candidate divisions. PLoS One 2013;8:e63994 [CrossRef][PubMed]
    [Google Scholar]
  8. Moore EK, Villanueva L, Hopmans EC, Rijpstra WI, Mets A et al. Abundant trimethylornithine lipids and specific gene sequences are indicative of planctomycete importance at the oxic/anoxic interface in Sphagnum-dominated northern wetlands. Appl Environ Microbiol 2015;81:6333–6344 [CrossRef][PubMed]
    [Google Scholar]
  9. Ivanova AA, Naumoff DG, Miroshnikov KK, Liesack W, Dedysh SN. Comparative genomics of four Isosphaeraceae planctomycetes: a common pool of plasmids and glycoside hydrolase genes shared by Paludisphaera borealis PX4T, Isosphaera pallida IS1BT, Singulisphaera acidiphila DSM 18658T, and Strain SH-PL62. Front Microbiol 2017;8:412 [CrossRef][PubMed]
    [Google Scholar]
  10. Ivanova AA, Kulichevskaya IS, Merkel AY, Toshchakov SV, Dedysh SN. High diversity of planctomycetes in soils of two lichen-dominated sub-arctic ecosystems of northwestern Siberia. Front Microbiol 2016;7:7 [CrossRef][PubMed]
    [Google Scholar]
  11. Staley JT, Fuerst JA, Giovannoni S, Schlesner H. The order Planctomycetales and the genera Planctomyces, Pirellula, Gemmata and Isosphaera. In Balows A, Trüper H, Dworkin M, Harder W, Schleifer KH. (editors) The Prokaryotes: A Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd ed. New York: Springer; 1992; pp.3710–3731
    [Google Scholar]
  12. Gerhardt P. Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981
    [Google Scholar]
  13. Kulichevskaya IS, Ivanova AO, Belova SE, Baulina OI, Bodelier PL et al. Schlesneria paludicola gen. nov., sp. nov., the first acidophilic member of the order Planctomycetales, from Sphagnum-dominated boreal wetlands. Int J Syst Evol Microbiol 2007;57:2680–2687 [CrossRef][PubMed]
    [Google Scholar]
  14. Kulichevskaya IS, Baulina OI, Bodelier PL, Rijpstra WI, Damsté JS et al. Zavarzinella formosa gen. nov., sp. nov., a novel stalked, Gemmata-like planctomycete from a Siberian peat bog. Int J Syst Evol Microbiol 2009;59:357–364 [CrossRef][PubMed]
    [Google Scholar]
  15. Kulichevskaya IS, Serkebaeva YM, Kim Y, Rijpstra WI, Damsté JS et al. Telmatocola sphagniphila gen. nov., sp. nov., a novel dendriform planctomycete from northern wetlands. Front Microbiol 2012;3:146 [CrossRef][PubMed]
    [Google Scholar]
  16. Moore EK, Hopmans EC, Rijpstra WI, Villanueva L, Dedysh SN et al. Novel mono-, di-, and trimethylornithine membrane lipids in northern wetland planctomycetes. Appl Environ Microbiol 2013;79:6874–6884 [CrossRef][PubMed]
    [Google Scholar]
  17. Collins MD. Analysis of isoprenoid quinones. Methods Microbiol 1985;18:329–366[CrossRef]
    [Google Scholar]
  18. Ward N. Phylum XXV. Planctomycetes Garrity and Holt 2001, 137 emend. Ward. In Krieg NR, Staley JT, Brown DR, Hedlung BP, Paster BJ et al. (editors) Bergey’s Manual of Systematic Bacteriologyvol. 4 New York: Springer; 2010; pp.879–925[CrossRef]
    [Google Scholar]
  19. Owen RJ, Hill LR, Lapage SP. Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers 1969;7:503–516 [CrossRef][PubMed]
    [Google Scholar]
  20. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991;173:697–703 [CrossRef][PubMed]
    [Google Scholar]
  21. Ludwig W, Strunk O, Westram R, Richter L, Meier H et al. ARB: a software environment for sequence data. Nucleic Acids Res 2004;32:1363–1371 [CrossRef][PubMed]
    [Google Scholar]
  22. Felsenstein J. PHYLIP – phylogeny inference package (version 3.2). Cladistics 1989;5:164–166
    [Google Scholar]
  23. Scheuner C, Tindall BJ, Lu M, Nolan M, Lapidus A et al. Complete genome sequence of Planctomyces brasiliensis type strain (DSM 5305T), phylogenomic analysis and reclassification of Planctomycetes including the descriptions of Gimesia gen. nov., Planctopirus gen. nov. and Rubinisphaera gen. nov. and emended descriptions of the order Planctomycetales and the family Planctomycetaceae. Stand Genomic Sci 2014;9:10 [CrossRef][PubMed]
    [Google Scholar]
  24. Göker M, Cleland D, Saunders E, Lapidus A, Nolan M et al. Complete genome sequence of Isosphaera pallida type strain (IS1BT). Stand Genomic Sci 2011;4:63–71 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002172
Loading
/content/journal/ijsem/10.1099/ijsem.0.002172
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error