sp. nov., isolated from rhizosphere of mugunghwa () Free

Abstract

A Gram-stain-positive, aerobic, non-motile, short-rod shaped actinobacterium, designated THG-T2.14, was isolated from soil sampled from the rhizosphere of mugunghwa. Growth occurred at 10–40 °C (optimum 30 °C), at pH 5.0–10.0 (optimum 7.0) and at 0–7.0 % NaCl (optimum 3.0 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T2.14 were identified as DSM 23203 (98.8 %), DSM 8608 (98.8 %), DSM 22421 (98.7 %) and KACC 17124 (98.4 %). The major fatty acids were anteiso-C, anteiso-C and iso-C. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, one unidentified lipid, two unidentified phospholipids and two unidentified phosphoglycolipids. The menaquinones were MK-12, and MK-13. The major polyamine was spermidine. The peptidoglycan contained ornithine, alanine, glycine, homoserine and glutamic acid. The diagnostic diamino acid was ornithine. The acyl type of the muramic acid was glycolyl. The whole-cell sugars were rhamnose, ribose, galactose, arabinose, xylose and glucose. The DNA G+C content of strain THG-T2.14 was 71.2 mol%. The DNA–DNA relatedness between strain THG-T2.14 and its closest reference strains were significantly lower than the threshold value of 70 %. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain THG-T2.14 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is THG-T2.14 (=KACC 18931=CCTCC AB 2016180).

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2017-09-01
2024-03-29
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