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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 67, Issue 9

sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin Free

Abstract

A novel bacterial strain S36 was isolated from the deep-sea sediment collected from Pacmanus hydrothermal field, Manus Basin. The strain was Gram-stain-positive, aerobic, rod-shaped, endospore-forming, and motile. It was able to grow at 16–50 °C, pH 6.0–10.0, and in the presence of 0–11 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain S36 was a member of genus and shares the highest sequence identity with D-1,5a (97.0 %). The value of DNA–DNA hybridization between strain S36 and D-1,5a was 22.8 %. The cell wall diagnostic diamino acid of strain S36 was -diaminopimelic acid and the polar lipid profile of strain S36 contained diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The predominant respiratory quinine was MK-7. The major cellular fatty acids were iso-C and anteiso-C. The genomic DNA G+C content of strain S36 was 43.0 mol%. On the basis of phylogenetic analysis, DNA–DNA hybridization, and phenotypic characteristics, it was concluded that strain S36 represents a novel species of the genus , for which the name sp. nov. was proposed. The type strain is S36 (=KCTC 33864=DSM 104297=CGMCC 1.16030).

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Keyword(s): Bacillus sp. nov. , hydrothermal sediment , taxonomy and type strain
© 2017 The copyright of this article belongs to Institute of Oceanology
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2017-09-01
2025-04-29
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Bacillus iocasae sp. nov., isolated from Pacmanus hydrothermal field, Manus Basin
Int J Syst Evol Microbiol 67, 3547 (2017); https://doi.org/10.1099/ijsem.0.002164
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