1887

Abstract

Two bacterial strains, EAod9 and SMJ21, isolated from salt-marsh plants, were determined to be related to species of the genus from from 16S rRNA sequence comparisons. Their closest phylogenetic relatives are members of the Gazogenes clade, and , which show the greatest similarity to the SMJ21rRNA sequence (97.3 and 97.1 %, respectively), while EAod9 had less than 97.0 % similarity to any other species of the genus . Both strains share the basic characteristics of the genus , as they are Gram-stain negative, motile, slightly halophilic, facultatively anaerobic bacteria. In addition, they are oxidase-negative and unable to grow on TCBS Agar; they grow between 15 to 26 °C, pH 6 to 8 and in up to 10 % (w/v) total salinity. They produce indol, are positive in the Voges-Proskauer test and are negative for arginine dihydrolase, lysine and ornithine decarboxylases. Strain SMJ21 is aerogenic and red-pigmented, due to prodigiosin production, while strain EAod9 ferments glucose without gas and is not pigmented. The major cellular fatty acids of both novel strains were Cω7/C ω6 and C. WGSobtained for both strains, along with the other five members of the clade, allowed the determination of ANI indexes and estimations of DDH values, which confirmed that the two strains represent two novel species of the genus : sp. nov. (with EAod9=CECT 9027=LMG 29724 as the proposed type strain) and sp. nov. (with SMJ21=CECT 9026=LMG 29723 as the proposed type strain).

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2017-09-01
2019-12-14
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