1887

Abstract

A Gram-stain-negative strain, A60, isolated from a water well sample in Portugal, was characterized phenotypically, genotypically and phylogenetically. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A60 belonged to the genus , and gene phylogeny revealed one strongly supported clade encompassing strain A60 and 13 other strains from public databases, distinct from currently recognized species of the genus . Furthermore, multilocus sequence analysis (MLSA) based on concatenated partial , , and sequences confirmed the classification obtained with the sequence. genomic comparisons, including average nucleotide identity (ANI) and the genome-to-genome distance calculator (GGDC), showed 94.6 % and 58.4 % identity to the closest relative ATCC 8090, respectively. The ability to metabolize different compounds further discriminated strain A60 from other species of the genus . The G+C content of strain A60 is 52.0 %. The results obtained support the description of a novel species within the genus , for which the name sp. nov. is proposed, with the type strain A60 (=DSM 104542=CECT 9236).

Keyword(s): 16S rRNA , ANI , Citrobacter freundii , GGDC , MLSA and recN
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2017-09-01
2024-03-28
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