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Abstract

A novel Gram-stain-negative, rod-shaped and non-motile bacterial strain, designated strain Seoho-37, was isolated from a eutrophic lake in South Korea. Polyphasic studies were performed to investigate the taxonomic position of the new isolate. The isolate grew aerobically with 0–1.0 % (w/v) NaCl (optimum 0 %), at pH 6.0–10.0 (optimum pH 7.0–9.0) and at temperatures of 15–36 °C (optimum 25–30 °C) on R2A medium. In the phylogenetic analysis of 16S rRNA gene sequences, strain Seoho-37 formed a clear cluster with the strains of Reyranella graminifolii , Reyranella massiliensis and Reyranella soli with a bootstrap resampling value of 100 %. DNA–DNA relatedness between strain Seoho-37 and the type strains of each species in the genus Reyranella was <20 %. The genomic DNA G+C content of strain Seoho-37 was 66.5 mol%. Ubiquinone-10 (Q-10) and ubiquinone-9 (Q-9) were found as the respiratory quinones. The cellular polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol and phosphatidylmethylethanolamine. The major fatty acid components included C16 : 0, summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and C18 : 1 2-OH. Based on the above evidence from a polyphasic study, strain Seaho-37 represents a novel species of the genus Reyranella , for which the name Reyranella aquatilis sp. nov. is proposed. The type strain is Seoho-37 (=KCTC 52223=JCM 31892).

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2017-09-06
2019-10-16
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