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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 9

sp. nov., a novel alkaliphilic and moderately halophilic bacterium isolated from soda soil Free

Abstract

An alkaliphilic and moderately halophilic strain characterized by optimal growth at pH 9.0–10.0 and 7 % (w/v) NaCl, and designated B16-24, was isolated from the rhizosphere soil of the bayonet grass at a soda pond in the Kiskunság National Park, Hungary. Cells of the strain were Gram-staining-positive, non-motile, straight rods, and formed central, ellipsoidal endospores with slightly swollen sporangia. The isolate was facultative anaerobic, catalase positive, oxidase negative, and contained a peptidoglycan of type A1γ based on -diaminopimelic acid. Menaquinone-7 (MK-7) was the predominant isoprenoid quinone, and anteiso-C the major cellular fatty acid. The DNA G+C content of strain B16-24 was 36.6 mol%. The 16S rRNA gene-based phylogenetic analysis revealed that the novel isolate had the greatest similarities to the type strains of Kh10-101 (97.8 %), 1139 (97.4 %), K1-25 (97.3 %) and N-1 (97.1 %). The DNA–DNA relatedness of strain B16-24 and the closely related species ranged between 24±6 % and 35±3 %. The distinctive phenotypic and genetic results of this study confirmed that strain B16-24 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is B16-24 (=DSM 29791=NCAIM B.02610).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): alkaliphilic , halophilic and soda soil
© 2017 IUMS
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2017-09-01
2024-04-19
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Bacillus kiskunsagensis sp. nov., a novel alkaliphilic and moderately halophilic bacterium isolated from soda soil
Int J Syst Evol Microbiol 67, 3490 (2017); https://doi.org/10.1099/ijsem.0.002149
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