1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 9

sp. nov., isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Free

Abstract

A Gram-stain-negative, non-motile, aerobic and ovoid or rod-shaped bacterium, designated JSSK-17, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain JSSK-17 grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 1.0–2.0 % (w/v) NaCl. Phylogenetic trees, based on 16S rRNA gene sequences, showed that strain JSSK-17 clustered with the type strains of and , exhibiting sequence similarities of 98.1–98.2 %. Strain JSSK-17 and the type strains of and contained Q-10 as the predominant ubiquinone and Cω7 as the major fatty acid. The predominant polar lipids detected in strain JSSK-17 and the type strains of and were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminolipid, one unidentified glycolipid and one unidentified phospholipid. The DNA G+C content of strain JSSK-17 was 64.4 mol% and its DNA–DNA relatedness values with the type strains of and were 24 and 15 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain JSSK-17 is separated from the two reference species of the genus . On the basis of the data presented, strain JSSK-17 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JSSK-17 (=KCTC 52765=NBRC 112766).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): freshwater spring , novel species , polyphasic taxonomy , Puniceibacterium confluentis sp. nov and the ocean
© 2017 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002145
2017-09-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/9/3480.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002145&mimeType=html&fmt=ahah

References

  1. Liu C, Zhang XY, Su HN, Zhou MY, Chen B et al. Puniceibacterium antarcticum gen. nov., sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2014; 64:1566–1572 [View Article][PubMed]
     [Google Scholar]
  2. Zhang DC, Neuner K, Wu J, Yao J, Margesin R. Puniceibacterium sediminis sp. nov., from intertidal sediment. Int J Syst Evol Microbiol 2015; 65:1462–1466 [View Article][PubMed]
     [Google Scholar]
  3. Park S, Won SM, Kim H, Park DS, Yoon JH. Aestuariivita boseongensis gen. nov., sp. nov., isolated from a tidal flat sediment. Int J Syst Evol Microbiol 2014; 64:2969–2974 [View Article][PubMed]
     [Google Scholar]
  4. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987; 19:1–67
     [Google Scholar]
  5. Barrow GI, Feltham RKA. Cowan and Steel’s Manual for the Identification of Medical Bacteria, 3rd ed. Cambridge: Cambridge University Press; 1993 [CrossRef]
     [Google Scholar]
  6. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001; 51:1997–2006 [View Article][PubMed]
     [Google Scholar]
  7. Baumann P, Baumann L. The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG et al. (editors) The Prokaryotes Berlin: Springer; 1981 pp. 1302–1331
     [Google Scholar]
  8. Cohen-Bazire G, Sistrom WR, Stanier RY. Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Comp Physiol 1957; 49:25–68 [View Article][PubMed]
     [Google Scholar]
  9. Staley JT. Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 1968; 95:1921–1942[PubMed]
     [Google Scholar]
  10. Rainey FA, Silva J, Nobre MF, Silva MT, da Costa MS. Porphyrobacter cryptus sp. nov., a novel slightly thermophilic, aerobic, bacteriochlorophyll a-containing species. Int J Syst Evol Microbiol 2003; 53:35–41 [View Article][PubMed]
     [Google Scholar]
  11. Yoon J-H, Kim H, Kim S-B, Kim H-J, Kim WY et al. Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 1996; 46:502–505 [View Article]
     [Google Scholar]
  12. Yoon J-H, Lee ST, Kim S-B, Kim WY, Goodfellow M et al. Restriction fragment length polymorphism analysis of PCR-amplified 16S ribosomal DNA for rapid identification of Saccharomonospora strains. Int J Syst Bacteriol 1997; 47:111–114 [View Article]
     [Google Scholar]
  13. Yoon JH, Kim IG, Shin DY, Kang KH, Park YH et al. Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 2003; 53:53–57 [View Article][PubMed]
     [Google Scholar]
  14. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
     [Google Scholar]
  15. Komagata K, Suzuki K. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 1987; 19:161–207 [CrossRef]
     [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  17. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  18. Embley TM, Wait R. Structural lipids of eubacteria. In Goodfellow M, O’Donnell AG. (editors) Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics Chichester: John Wiley & Sons; 1994 pp. 121–161
     [Google Scholar]
  19. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
     [Google Scholar]
  20. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
     [Google Scholar]
  21. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
     [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.002145
Loading
Puniceibacterium confluentis sp. nov., isolated from the junction between the ocean and a freshwater spring, and emended description of the genus Puniceibacterium
Int J Syst Evol Microbiol 67, 3480 (2017); https://doi.org/10.1099/ijsem.0.002145
/content/journal/ijsem/10.1099/ijsem.0.002145
/content/journal/ijsem/10.1099/ijsem.0.002145
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error