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Volume 67, Issue 9

gen. nov., sp. nov., a thermophilic, chemolithoautotrophic bacterium capable of respiratory ammonification of nitrate with elemental sulfur Free

Abstract

An extremely thermophilic, anaerobic, chemolithoautotrophic bacterium (strain ST65) was isolated from a deep-sea hydrothermal vent chimney located on the Eastern Lau Spreading Centre in the south-western Pacific Ocean, at a depth of 1870 m. Cells of strain ST65 were non-motile straight or slightly curved short rods, 0.5–0.6 µm in diameter and 0.8–1.5 µm in length. The temperature range for growth was 47–75 °C, with an optimum at 65 °C. The pH range for growth was 5.5–7.5, with an optimum at pH 6.5. Growth of strain ST65 was observed at NaCl concentrations ranging from 1.5 to 4.5 % (w/v), with an optimum at 2.0–2.5 %. Strain ST65 grew anaerobically with inorganic carbon as a carbon source and with elemental sulfur as an electron donor and nitrate as an electron acceptor producing sulfate and ammonium. It was also able to grow by disproportionation of elemental sulfur, thiosulfate and sulfite. Sulfate was not utilized as an electron acceptor. Analysis of the 16S rRNA gene sequence revealed that the isolate belongs to a deep lineage in the phylum . On the basis of its physiological properties and results of phylogenetic analyses, it is proposed that the isolate represents a novel species of a new genus, gen. nov., sp. nov. ST65 (=DSM 102941=VKM B-2855) is the type strain of the type species.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): deep-sea micro-organisms , DNRA , microbial sulfur oxidation , nitrate reduction , respiratory ammonification , Thermodesulfobacteria and thermophiles
© 2017 IUMS
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2017-09-01
2024-04-19
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Thermosulfuriphilus ammonigenes gen. nov., sp. nov., a thermophilic, chemolithoautotrophic bacterium capable of respiratory ammonification of nitrate with elemental sulfur
Int J Syst Evol Microbiol 67, 3474 (2017); https://doi.org/10.1099/ijsem.0.002142
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