RT Journal Article SR Electronic(1) A1 Wang, Dandan A1 Wei, Yuxi A1 Cui, Qiu A1 Li, WenliYR 2017 T1 Amylibacter cionae sp. nov., isolated from the sea squirt Ciona savignyi JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 9 SP 3462 OP 3466 DO https://doi.org/10.1099/ijsem.0.002140 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, non-motile, aerobic and rod-shaped bacterial strain, designated H-12T, was isolated from a sea squirt (Ciona savignyi) collected from Tsingtao Port, Jiaozhou Bay, China, and its taxonomic position was investigated. Strain H-12T grew optimally at 25–30 °C, at pH 7.0–8.0 and in the presence of 3.0–4.0 % (w/v) NaCl. The 16S rRNA gene sequence of strain H-12T exhibited the highest similarity to that of the type strain of Amylibacter marinus (95.3 %). A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain H-12T clustered with the type strain of A. marinus . The predominant ubiquinone in strain H-12T was identified as Q-10. The major fatty acids of strain H-12T were C18 : 1ω7c and C18 : 1ω7c 11-methyl. The major polar lipids detected in strain H-12T were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, one unidentified aminolipid, two unidentified phospholipids and five unidentified lipids. The DNA G+C content of strain H-12T was 52.7 mol%. On the basis of phylogenetic, chemotaxonomic and phenotypic properties, strain H-12T is considered to represent a novel species within the genus Amylibacter , for which the name Amylibacter cionae sp. nov. is proposed. The type strain is H-12T (=KCTC 52581T=CGMCC 1.15880T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002140