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Abstract

A novel Gram-staining-negative, spiral-shaped, pale-yellow, non-sporulating, motile, aerobic bacterium, designated strain AK56, was isolated from a sediment sample collected at the Coringa Wildlife Sanctuary, India. Colonies on marine agar were circular, pale yellow, shiny, translucent, 1–2 mm in diameter, convex and had an entire margin. The major fatty acids included C16 : 1, C16 : 1 ω7 c/C16 : 1ω6c and C18 : 1ω7c. Polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, one unidentified phospholipid and five unidentified lipids. DNA–DNA hybridization between strain AK56 and Oceanospirillum linum LMG 5214 and ‘ Oceanospirillum nioense ’ NIO-S6 showed relatedness values of 39.91 and 23.62 %, respectively. The DNA G+C content of strain AK56 was found to be 50.3 mol%. A sequence similarity search for the 16S rRNA gene sequence revealed that O. linum and O. nioense were the nearest phylogenetic neighbours, with a pair-wise sequence similarity of 98.9 and 98.2 %, respectively. Phylogenetic analysis also showed the formation of a cluster including strain AK56 with close relative O. linum and O. nioense . Based on the observed phenotypic, chemotaxonomic characteristics and phylogenetic analysis, strain AK56 is described in this study as a novel species in the genus Oceanospirillum , for which the name Oceanospirillum sanctuarii sp. nov. is proposed. The type strain of Oceanospirillum sanctuarii is AK56 (=MTCC 12005=JCM 19193=KCTC 52973).

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2017-08-22
2019-10-23
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References

  1. Williams MA, Rittenberg SC. A taxonomic study of the genus Spirillum Ehrenberg. Int Bull Bacteriol Nomencl Taxon 1957;7:49–112 [CrossRef]
    [Google Scholar]
  2. Garrity GM, Bell JA, Lilburn T. Family I. Oceanospirillaceae fam. nov. Bergey's Manual Syst Bacteriol 2 (Part B) Springer; 2005; pp.271
    [Google Scholar]
  3. Hedlund BP, Geiselbrecht AD, Bair TJ, Staley JT. Polycyclic aromatic hydrocarbon degradation by a new marine bacterium, Neptunomonas naphthovorans gen. nov., sp. nov. Appl Environ Microbiol 1999;65:251–259[PubMed]
    [Google Scholar]
  4. Dimitriu PA, Shukla SK, Conradt J, Márquez MC, Ventosa A et al. Nitrincola lacisaponensis gen. nov., sp. nov., a novel alkaliphilic bacterium isolated from an alkaline, saline lake. Int J Syst Evol Microbiol 2005;55:2273–2278 [CrossRef][PubMed]
    [Google Scholar]
  5. Macián MC, Arahal DR, Garay E, Pujalte MJ. Marinomonas aquamarina sp. nov., isolated from oysters and seawater. Syst Appl Microbiol 2005;28:145–150 [CrossRef][PubMed]
    [Google Scholar]
  6. Arahal DR, Lekunberri I, González JM, Pascual J, Pujalte MJ et al. Neptuniibacter caesariensis gen. nov., sp. nov., a novel marine genome-sequenced gammaproteobacterium. Int J Syst Evol Microbiol 2007;57:1000–1006 [CrossRef][PubMed]
    [Google Scholar]
  7. Gärtner A, Wiese J, Imhoff JF. Amphritea atlantica gen. nov., sp. nov., a gammaproteobacterium from the logatchev hydrothermal vent field. Int J Syst Evol Microbiol 2008;58:34–39 [CrossRef][PubMed]
    [Google Scholar]
  8. Miyazaki M, Nogi Y, Fujiwara Y, Kawato M, Kubokawa K et al. Neptunomonas japonica sp. nov., an Osedax japonicus symbiont-like bacterium isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan. Int J Syst Evol Microbiol 2008;58:866–871 [CrossRef][PubMed]
    [Google Scholar]
  9. Schlösser A, Lipski A, Schmalfuss J, Kugler F, Beckmann G. Oceaniserpentilla haliotis gen. nov., sp. nov., a marine bacterium isolated from haemolymph serum of blacklip abalone. Int J Syst Evol Microbiol 2008;58:2122–2125 [CrossRef][PubMed]
    [Google Scholar]
  10. Pinhassi J, Pujalte MJ, Pascual J, González JM, Lekunberri I et al. Bermanella marisrubri gen. nov., sp. nov., a genome-sequenced gammaproteobacterium from the Red Sea. Int J Syst Evol Microbiol 2009;59:373–377 [CrossRef][PubMed]
    [Google Scholar]
  11. Teramoto M, Ohuchi M, Hatmanti A, Darmayati Y, Widyastuti Y et al. Oleibacter marinus gen. nov., sp. nov., a bacterium that degrades petroleum aliphatic hydrocarbons in a tropical marine environment. Int J Syst Evol Microbiol 2011;61:375–380 [CrossRef][PubMed]
    [Google Scholar]
  12. Chen MH, Sheu SY, Chen CA, Wang JT, Chen WM. Corallomonas stylophorae gen. nov., sp. nov., a halophilic bacterium isolated from the reef-building coral Stylophora pistillata. Int J Syst Evol Microbiol 2013;63:982–988 [CrossRef][PubMed]
    [Google Scholar]
  13. Dauga C, Gillis M, Vandamme P, Ageron E, Grimont F et al. Balneatrix alpica gen. nov., sp. nov., a bacterium associated with pneumonia and meningitis in a spa therapy center. Res Microbiol 1993;144:35–46[PubMed][CrossRef]
    [Google Scholar]
  14. Satomi M, Kimura B, Hamada T, Harayama S, Fujii T. Phylogenetic study of the genus Oceanospirillum based on 16S rRNA and gyrB genes: emended description of the genus Oceanospirillum, description of Pseudospirillum gen. nov., Oceanobacter gen. nov. and Terasakiella gen. nov. and transfer of Oceanospirillum jannaschii and Pseudomonas stanieri to Marinobacterium as Marinobacterium jannaschii comb. nov. and Marinobacterium stanieri comb. no. Int J Syst Evol Microbiol 2002;52:739–747 [CrossRef][PubMed]
    [Google Scholar]
  15. Vandamme P, Pot B, Gillis M, de Vos P, Kersters K et al. Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 1996;60:407–438[PubMed]
    [Google Scholar]
  16. Bernardet JF, Nakagawa Y, Holmes B.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002;52:1049–1070 [CrossRef][PubMed]
    [Google Scholar]
  17. Lányí B. Classical and rapid identification methods for medically important bacteria. Methods Microbiol 1987;19:1–67
    [Google Scholar]
  18. Cowan ST, Steel KJ. Manual for the Identification of Medical Bacteria London: Cambridge University Press; 1965
    [Google Scholar]
  19. Gordon RE, Barnett DA, Handerhan JE, Pang CH-N. Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 1974;24:54–63 [CrossRef]
    [Google Scholar]
  20. Baek SH, Cui Y, Kim SC, Cui CH, Yin C et al. Tumebacillus ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2011;61:1715–1719[CrossRef]
    [Google Scholar]
  21. Sasser M. Identification of bacteria through fatty acid analysis. In Klement Z, Rudolph K, Sands Budapest DC. (editors) Methods in Phytobacteriology Hungary: Akademiai Kiado; 1990; pp.199–204
    [Google Scholar]
  22. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959;37:911–917[CrossRef]
    [Google Scholar]
  23. Komagata K, Suzuki K. Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 1987;19:161–207[CrossRef]
    [Google Scholar]
  24. Surendra V, Bhawana P, Suresh K, Srinivas TNR, Kumar PA. Imtechella halotolerans gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from estuarine water. Int J Syst Evol Microbiol 2012;62:2624–2630[CrossRef]
    [Google Scholar]
  25. Sly LI, Blackall LL, Kraat PC, Tao TS, Sangkhobol V. The use of second derivative plots for the determination of mol% guanine plus cytosine of DNA by the thermal denaturation method. J Microbiol Methods 1986;5:139–156[CrossRef]
    [Google Scholar]
  26. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  27. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739[CrossRef]
    [Google Scholar]
  28. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120[CrossRef]
    [Google Scholar]
  29. Tourova TP, Antonov AS. Identification of microorganisms by rapid DNA–DNA hybridization. Method Microbiol 1988;19:333–355[CrossRef]
    [Google Scholar]
  30. Rakshak K, Ravinder K, Nupur STNR, Anil Kumar P. Caldimonas meghalayensis sp. nov., a novel thermophilic betaproteobacterium isolated from a hot spring of Meghalaya in northeast India. Antonie van Leeuwenhoek 2013;104:1217–1225[CrossRef]
    [Google Scholar]
  31. Pot B, Gillis M..Genus I. Oceanospirillum Bergey's Manual Syst Bacteriol 2 (Part B) Springer; 2005; pp.271–281
    [Google Scholar]
  32. Krishna KK, Bhumika V, Thomas M, Anil Kumar P, Srinivas TN. Oceanospirillum nioense sp. nov., a marine bacterium isolated from sediment sample of Palk bay, India. Antonie van Leeuwenhoek 2013;103:1015–1021 [CrossRef][PubMed]
    [Google Scholar]
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