1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 9

Description of gen. nov., sp. nov., an obligately anaerobic bacterium isolated from coastal marine sediments, and reclassification of as comb. nov. Free

Abstract

An obligately anaerobic spirochaete (strain SY2) was isolated from coastal marine sediments of Tongyeong-Si, South Korea. Strain SY2 was helical-shaped and Gram-stain-negative. Strain SY2 was able to grow at 10–40 °C (optima, 25–30 °C), pH 6.3–8.8 (optima, pH 7.0–8.0) and with 1–7 % (optimum, 2–3 %) NaCl concentration. Strain SY2 was negative for catalase and oxidase activity. The major end-products of glucose fermentation were acetate, ethanol, hydrogen and carbon dioxide. C, C, iso-C, iso-C 3-OH, iso-C H/C 3-OH and iso-Cω9 were predominant fatty acids (>5 %) with minor amounts (<5 %) of C, iso-C, iso-C, iso-C/anteiso-C B and Cω6/C ω7. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were major polar lipids. The genomic DNA G+C content was 53.5 mol%. 16S rRNA gene sequence comparisons indicated that strain SY2 represents a member of the family in the phylum . Strain SY2 has a sequence similarity of 95.1 % with R1 and <90.1 % with other members of the genus . Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain SY2 as a representative of a novel genus and species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is SY2 (=KEMB 3001-381=DSM 104770=KCTC 15593). Reclassification of as comb. nov. is also proposed based on polyphasic taxonomic analyses.

  • Received:
  • Accepted:
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Keyword(s): coastal marine sediments , novel genus , Oceanispirochaeta sediminicola and Spirochaetaceae
© 2017 IUMS
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2017-09-01
2023-04-02
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Description of Oceanispirochaeta sediminicola gen. nov., sp. nov., an obligately anaerobic bacterium isolated from coastal marine sediments, and reclassification of Spirochaeta litoralis as Oceanispirochaeta litoralis comb. nov.
Int J Syst Evol Microbiol 67, 3403 (2017); https://doi.org/10.1099/ijsem.0.002130
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