1887

Abstract

An obligately anaerobic spirochaete (strain SY2) was isolated from coastal marine sediments of Tongyeong-Si, South Korea. Strain SY2 was helical-shaped and Gram-stain-negative. Strain SY2 was able to grow at 10–40 °C (optima, 25–30 °C), pH 6.3–8.8 (optima, pH 7.0–8.0) and with 1–7 % (optimum, 2–3 %) NaCl concentration. Strain SY2 was negative for catalase and oxidase activity. The major end-products of glucose fermentation were acetate, ethanol, hydrogen and carbon dioxide. C14 : 0, C16 : 0, iso-C15 : 0, iso-C14 : 0 3-OH, iso-C15 : 1 H/C13 : 0 3-OH and iso-C17 : 1ω9c were predominant fatty acids (>5 %) with minor amounts (<5 %) of C18 : 0, iso-C13 : 0, iso-C17 : 0, iso-C17 : 1/anteiso-C17 : 1 B and C16 : 1ω6c/C16  : 1ω7c. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were major polar lipids. The genomic DNA G+C content was 53.5 mol%. 16S rRNA gene sequence comparisons indicated that strain SY2 represents a member of the family Spirochaetaceae in the phylum Spirochaetes . Strain SY2 has a sequence similarity of 95.1 % with Spirochaeta litoralis R1 and <90.1 % with other members of the genus Spirochaeta . Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain SY2 as a representative of a novel genus and species in the family Spirochaetaceae , for which the name Oceanispirochaeta sediminicola gen. nov., sp. nov. is proposed. The type strain is SY2 (=KEMB 3001-381=DSM 104770=KCTC 15593). Reclassification of Spirochaeta litoralis as Oceanispirochaeta litoralis comb. nov. is also proposed based on polyphasic taxonomic analyses.

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2017-09-06
2019-10-22
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References

  1. Aksenova HY, Rainey FA, Janssen PH, Zavarzin GA, Morgan HW. Spirochaeta thermophila sp. nov., an obligately anaerobic, polysaccharolytic, extremely thermophilic bacterium. Int J Syst Bacteriol 1992;42:175–177 [CrossRef]
    [Google Scholar]
  2. Arroua B, Ranchou-Peyruse A, Ranchou-Peyruse M, Magot M, Urios L et al. Pleomorphochaeta caudata gen. nov., sp. nov., an anaerobic bacterium isolated from an offshore oil well, reclassification of Sphaerochaeta multiformis MO-SPC2T as Pleomorphochaeta multiformis MO-SPC2T comb. nov. as the type strain of this novel genus and emended description of the genus Sphaerochaeta. Int J Syst Evol Microbiol 2017;67:417–424 [CrossRef][PubMed]
    [Google Scholar]
  3. Breznak JA, Canale-Parola E. Morphology and physiology of Spirochaeta aurantia strains isolated from aquatic habitats. Arch Microbiol 1975;105:1–12 [CrossRef][PubMed]
    [Google Scholar]
  4. Breznak JA, Warnecke F. Spirochaeta cellobiosiphila sp. nov., a facultatively anaerobic, marine spirochaete. Int J Syst Evol Microbiol 2008;58:2762–2768 [CrossRef][PubMed]
    [Google Scholar]
  5. Campbell BJ, Cary SC. Characterization of a novel spirochete associated with the hydrothermal vent polychaete annelid, Alvinella pompejana. Appl Environ Microbiol 2001;67:110–117 [CrossRef][PubMed]
    [Google Scholar]
  6. Canale-Parola E. Free-living saccharolytic spirochetes: the genus Spirochaeta. In Balows A, Truper HG, Dworkin M, Harder W, Schleifer K-H et al. (editors) The Prokaryotes, 2nd ed. New York: Springer-Verlag; 1992; pp.3524–3536[CrossRef]
    [Google Scholar]
  7. Canale-Parola E. Genus I. Spirochaeta. Ehrenberg 1835, 313AL. In Krieg NR, Holt JG. (editors) Bergey’s Manual of Systematic Bacteriologyvol. 1 Baltimore: Williams & Wilkins; 1984; pp.39–46
    [Google Scholar]
  8. Canale-Parola E. Revival of the names Spirochaeta litoralis, Spirochaeta zuelzerae, and Spirochaeta aurantia. Int J Syst Bacteriol 1980;30:594 [CrossRef]
    [Google Scholar]
  9. Dröge S, Fröhlich J, Radek R, König H. Spirochaeta coccoides sp. nov., a novel coccoid spirochete from the hindgut of the termite Neotermes castaneus. Appl Environ Microbiol 2006;72:392–397 [CrossRef][PubMed]
    [Google Scholar]
  10. Dubinina G, Grabovich M, Leshcheva N, Rainey FA, Gavrish E et al. Spirochaeta perfilievii sp. nov., an oxygen-tolerant, sulfide-oxidizing, sulfur- and thiosulfate-reducing spirochaete isolated from a saline spring. Int J Syst Evol Microbiol 2011;61:110–117 [CrossRef][PubMed]
    [Google Scholar]
  11. Dubinina G, Grabovich M, Leshcheva N, Gronow S, Gavrish E et al. Spirochaeta sinaica sp. nov., a halophilic spirochaete isolated from a cyanobacterial mat. Int J Syst Evol Microbiol 2015;65:3872–3877 [CrossRef][PubMed]
    [Google Scholar]
  12. Evans NJ, Brown JM, Demirkan I, Murray RD, Birtles RJ et al. Treponema pedis sp. nov., a spirochaete isolated from bovine digital dermatitis lesions. Int J Syst Evol Microbiol 2009;59:987–991 [CrossRef][PubMed]
    [Google Scholar]
  13. Fracek SP, Stolz JF. Spirochaeta bajacaliforniensis sp. n. from a microbial mat community at Laguna Figueroa, Baja California Norte, Mexico. Arch Microbiol 1985;142:317–325 [CrossRef][PubMed]
    [Google Scholar]
  14. Greenberg EP, Canale-Parola E. Spirochaeta halophila sp. n., a facultative anaerobe from a high-salinity pond. Arch Microbiol 1976;110:185–194 [CrossRef][PubMed]
    [Google Scholar]
  15. Ben Hania W, Joseph M, Schumann P, Bunk B, Fiebig A et al. Complete genome sequence and description of Salinispira pacifica gen. nov., sp. nov., a novel spirochaete isolated form a hypersaline microbial mat. Stand Genomic Sci 2015;10:7 [CrossRef][PubMed]
    [Google Scholar]
  16. Harwood CS, Canale-Parola E. Spirochaeta isovalerica sp. nov., a marine anaerobe that forms branched-chain fatty acids as fermentation products. Int J Syst Bacteriol 1983;33:573–579 [CrossRef]
    [Google Scholar]
  17. Hespell RB, Canale-Parola E. Spirochaeta litoralis sp. n., a strictly anaerobic marine spirochete. Arch Microbiol 1970;74:1–18 [CrossRef]
    [Google Scholar]
  18. Imachi H, Sakai S, Hirayama H, Nakagawa S, Nunoura T et al. Exilispira thermophila gen. nov., sp. nov., an anaerobic, thermophilic spirochaete isolated from a deep-sea hydrothermal vent chimney. Int J Syst Evol Microbiol 2008;58:2258–2265 [CrossRef][PubMed]
    [Google Scholar]
  19. Koelschbach JS, Mouttaki H, Pickl C, Heipieper HJ, Rachel R et al. Rectinema cohabitans gen. nov., sp. nov., a rod-shaped spirochaete isolated from an anaerobic naphthalene-degrading enrichment culture. Int J Syst Evol Microbiol 2017;67:1288–1295 [CrossRef][PubMed]
    [Google Scholar]
  20. Leschine SB, Paster BJ. Genus Spirochaeta. In Krieg NR, Staley JM, Brown DR, Hedlund BP, Paster BJ et al. (editors) Bergey’s Manual of Systematic Bacteriology, 2nd ed.vol.4 New York: Springer; 2010; pp.473–484
    [Google Scholar]
  21. Magot M, Fardeau ML, Arnauld O, Lanau C, Ollivier B et al. Spirochaeta smaragdinae sp. nov., a new mesophilic strictly anaerobic spirochete from an oil field. FEMS Microbiol Lett 1997;155:185–191 [CrossRef][PubMed]
    [Google Scholar]
  22. Miyazaki M, Sakai S, Yamanaka Y, Saito Y, Takai K et al. Spirochaeta psychrophila sp. nov., a psychrophilic spirochaete isolated from subseafloor sediment, and emended description of the genus Spirochaeta. Int J Syst Evol Microbiol 2014;64:2798–2804 [CrossRef][PubMed]
    [Google Scholar]
  23. Pikuta EV, Hoover RB, Bej AK, Marsic D, Whitman WB et al. Spirochaeta dissipatitropha sp. nov., an alkaliphilic, obligately anaerobic bacterium, and emended description of the genus Spirochaeta Ehrenberg 1835. Int J Syst Evol Microbiol 2009;59:1798–1804 [CrossRef][PubMed]
    [Google Scholar]
  24. Pohlschroeder M, Leschine SB, Canale-Parola E. Spirochaeta caldaria sp. nov., a thermophilic bacterium that enhances cellulose degradation by Clostridium thermocellum. Arch Microbiol 1994;161:17–24 [CrossRef]
    [Google Scholar]
  25. Reddy SV, Aspana S, Tushar DL, Sasikala C, Ramana C. Spirochaeta sphaeroplastigenens sp. nov., a halo-alkaliphilic, obligately anaerobic spirochaete isolated from soda lake Lonar. Int J Syst Evol Microbiol 2013;63:2223–2228 [CrossRef][PubMed]
    [Google Scholar]
  26. Ritalahti KM, Justicia-Leon SD, Cusick KD, Ramos-Hernandez N, Rubin M et al. Sphaerochaeta globosa gen. nov., sp. nov. and Sphaerochaeta pleomorpha sp. nov., free-living, spherical spirochaetes. Int J Syst Evol Microbiol 2012;62:210–216 [CrossRef][PubMed]
    [Google Scholar]
  27. Schrank K, Choi BK, Grund S, Moter A, Heuner K et al. Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis. Int J Syst Bacteriol 1999;49:43–50 [CrossRef][PubMed]
    [Google Scholar]
  28. Shivani Y, Subhash Y, Sasikala C, Ramana CV. Description of 'Candidatus Marispirochaeta associata' and reclassification of Spirochaeta bajacaliforniensis, Spirochaeta smaragdinae and Spirochaeta sinaica to a new genus Sediminispirochaeta gen. nov. as Sediminispirochaeta bajacaliforniensis comb. nov., Sediminispirochaeta smaragdinae comb. nov. and Sediminispirochaeta sinaica comb. nov. Int J Syst Evol Microbiol 2016;66:5485–5492 [CrossRef][PubMed]
    [Google Scholar]
  29. Shivani Y, Subhash Y, Tushar L, Sasikala C, Ramana C. Spirochaeta lutea sp. nov., isolated from marine habitats and emended description of the genus Spirochaeta. Syst Appl Microbiol 2015;38:110–114 [CrossRef][PubMed]
    [Google Scholar]
  30. Sravanthi T, Tushar L, Sasikala C, Ramana ChV. Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as new combinations within Alkalispirochaeta gen. nov. Int J Syst Evol Microbiol 2016;66:1612–1619 [CrossRef][PubMed]
    [Google Scholar]
  31. Troshina O, Oshurkova V, Suzina N, Machulin A, Ariskina E et al. Sphaerochaeta associata sp. nov., a spherical spirochaete isolated from cultures of Methanosarcina mazei JL01. Int J Syst Evol Microbiol 2015;65:4315–4322 [CrossRef][PubMed]
    [Google Scholar]
  32. Umemoto T, Nakazawa F, Hoshino E, Okada K, Fukunaga M et al. Treponema medium sp. nov., isolated from human subgingival dental plaque. Int J Syst Bacteriol 1997;47:67–72 [CrossRef][PubMed]
    [Google Scholar]
  33. Wyss C, Choi BK, Schüpbach P, Guggenheim B, Göbel UB. Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int J Syst Bacteriol 1997;47:842–845 [CrossRef][PubMed]
    [Google Scholar]
  34. Wyss C, Dewhirst FE, Gmür R, Thurnheer T, Xue Y et al. Treponema parvum sp. nov., a small, glucoronic or galacturonic acid-dependent oral spirochaete from lesions of human periodontitis and acute necrotizing ulcerative gingivitis. Int J Syst Evol Microbiol 2001;51:955–962 [CrossRef][PubMed]
    [Google Scholar]
  35. Zhilina TN, Zavarzin GA, Rainey F, Kevbrin VV, Kostrikina NA et al. Spirochaeta alkalica sp. nov., Spirochaeta africana sp. nov., and Spirochaeta asiatica sp. nov., alkaliphilic anaerobes from the continental soda lakes in Central Asia and the East African Rift. Int J Syst Bacteriol 1996;46:305–312 [CrossRef][PubMed]
    [Google Scholar]
  36. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961;3:208–218 [CrossRef]
    [Google Scholar]
  37. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  38. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  39. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 2013;30:2725–2729 [CrossRef][PubMed]
    [Google Scholar]
  40. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  41. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  42. Oren A, Duker S, Ritter S. The polar lipid composition of walsby's square bacterium. FEMS Microbiol Lett 1996;138:135–140 [CrossRef]
    [Google Scholar]
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