1887

Abstract

An aerobic Gram-stain-negative prosthecate bacterium, designated RMAR8-3, was isolated from a marine red alga in the Republic of Korea. Cells were dimorphic rods with a single polar prostheca (non-motile) or flagellum (motile) showing catalase- and oxidase-positive reactions. Growth of strain RMAR8-3 was observed at 15–45 °C (optimum, 40 °C), at pH 6.0–9.0 (optimum, pH 7.0) and in the presence of 0–10 % (w/v) NaCl (optimum, 2 %). Ubiquinone-10 was detected as the sole isoprenoid quinone and C, summed feature 8 (comprising C 7 and/or Cω), C, C 3-OH and C were identified as the major cellular fatty acids. The major polar lipids were sulfo-quinovosyldiacylglycerol, glucuronopyranosyldiglyceride and monoglycosyldiglyceride. The G+C content of the genomic DNA was 66.3 mol%. Strain RMAR8-3 was most closely related to P-1 km-3 with a 97.6 % 16S rRNA gene sequence similarity. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain RMAR8-3 formed a tight phylogenic lineage with P-1 km-3 within the family . On the basis of phenotypic, chemotaxonomic and molecular features, strain RMAR8-3 clearly represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RMAR8-3 (=KACC 18990=JCM 31718).

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2017-09-01
2024-03-28
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References

  1. Zhang XY, Li GW, Wang CS, Zhang YJ, Xu XW et al. Marinicauda pacifica gen. nov., sp. nov., a prosthecate alphaproteobacterium of the family Hyphomonadaceae isolated from deep seawater. Int J Syst Evol Microbiol 2013; 63:2248–2253 [View Article][PubMed]
    [Google Scholar]
  2. Murphy CD, Moore RM, White RL. Peroxidases from marine microalgae. J Appl Phycol 2000; 12:507–513 [View Article]
    [Google Scholar]
  3. Kim JM, Le NT, Chung BS, Park JH, Bae JW et al. Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Appl Environ Microbiol 2008; 74:7313–7320 [View Article][PubMed]
    [Google Scholar]
  4. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: [View Article][PubMed]
    [Google Scholar]
  5. Nawrocki EP, Eddy SR. Query-dependent banding (QDB) for faster RNA similarity searches. PLoS Comput Biol 2007; 3:e56 [View Article][PubMed]
    [Google Scholar]
  6. Felsenstein J. PHYLIP (Phylogeny Inference Package) Version 3.695 Seattle, WA: Department of Genome Sciences, University of Washington;
    [Google Scholar]
  7. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary Genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016msw054
    [Google Scholar]
  8. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
  9. Rosselló-Móra R, Amann R. Past and future species definitions for Bacteria and Archaea. Syst Appl Microbiol 2015; 38:209–216 [View Article][PubMed]
    [Google Scholar]
  10. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 33:152–155
    [Google Scholar]
  11. Kim M, Oh HS, Park SC, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [View Article][PubMed]
    [Google Scholar]
  12. Smibert RM, Krieg NR. Phenotypic characterization. In Gerhardt P. (editor) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 607–654
    [Google Scholar]
  13. Lányí B. Classical and rapid identification methods for medically important Bacteria. Methods Microbiol 1988; 19:1–67 [CrossRef]
    [Google Scholar]
  14. Komagata K, Suzuki KI. Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 1988; 19:161–207 [CrossRef]
    [Google Scholar]
  15. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  16. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4:770–773[PubMed] [CrossRef]
    [Google Scholar]
  17. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117 [View Article]
    [Google Scholar]
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