1887

Abstract

Little is known on members of the genus inhabiting acidic habitats. In this study, a moderately acidophilic strain, designated 2-5, was isolated from coal mine soil, and subjected to a polyphasic taxonomic characterization. Analysis based on 16S rRNA gene sequences indicated that the strain was most closely related to the type strain of , sharing 99.30 % similarity, while similarity to all other species was less than 97 %. The DNA–DNA relatedness between the new isolate and the type strain of was 56.5±0.7 %. The optimal pH range of the isolate for growth was 5.5–6.0, but growth also occurred at pH 4.5 and 7.5. The isolate tolerated up to 6 % (w/v) NaCl (optimum, 0 %), and the temperature range for growth was 15–40 °C (optimum, 30 °C). The isolate was able to utilize most substrates tested for sole carbon sources, showing its metabolic versatility. The isolate exhibited antimicrobial activity against and weak antifungal activity against . The chemotaxonomic profiles of strain 2-5 included polar lipids containing phosphatidylethanolamine, phsphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol dimannosides, fatty acids containing Cω6 and iso-C as the major components, MK-9(H) as the predominant menaquinone, and -diaminopimelic acid and arabinose, galactose, glucose and ribose as the diagnostic diamino acid and sugars in the cell wall. The combined phenotypic, chemotaxonomic and genotypic analyses clearly indicated that the isolate merits recognition as represnting a novel species of , for which the name sp. nov. is proposed. The type strain is 2-5 (=KCTC 39523=JCM 30562).

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2017-09-01
2020-01-26
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