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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 9

sp. nov., a halophilic and thermotolerant crude-oil-degrading actinobacterium isolated from an oil-contaminated mud pit Free

Abstract

A crude-oil-degrading, Gram-stain-positive actinobacterial strain, RIPI, was isolated from a soil sample collected from an oil-contaminated mud pit in Khangiran oil and gas field, in the north-east of Iran. RIPI was strictly aerobic, catalase- and oxidase-positive. The strain grew with 0–12.5 % (w/v) NaCl (optimum 3–5 %), at 25–55 °C (optimum 45 °C) and at pH 6.0–9.5 (optimum pH 7.0). The results of 16S rRNA gene sequence comparative analysis indicated that RIPI represents a member of the genus , with high phylogenetic similarity to SCSIO 11529 (97.5 %), SP28S-3 (97.5 %) and MS498 (97.2 %). DNA–DNA relatedness values between the novel strain and DSM 45821, DSM 46655 and DSM 45268 were 28 , 19 and 23 %, respectively. The cell wall peptidoglycan of RIPI contained -diaminopimelic acid as the diamino acid and the whole-cell sugars are galactose and arabinose. The polar lipids pattern contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and two unknown phospholipids. Its cellular fatty acids pattern consisted of Cω6, iso-C and summed feature 3 (Cω7 and/or iso-C 2-OH), and the major respiratory quinone was MK-9(H). The G+C content of the genomic DNA was 69 mol%. On the basis of polyphasic taxonomic data we propose that RIPI represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is RIPI (=IBRC-M 10906=LMG 28389).

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  • Accepted:
  • Published Online:
Keyword(s): crude-oil-degrading , halophilic and thermotolerant
© 2017 IUMS
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2017-09-01
2024-04-25
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References

  1. Franco CMM, Labeda DP. The order Pseudonocardiales. In Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thampson F et al. (editors) The Prokaryotes–Actinobacteria, 4th ed. Berlin Heidelberg: Springer–Verlag; 2014 pp. 743–860
     [Google Scholar]
  2. Kim SB, Goodfellow M. Reclassification of Amycolatopsis rugosa Lechevalier et al. 1986 as Prauserella rugosa gen. nov., comb. nov. Int J Syst Bacteriol 1999; 49 Pt 2:507–512 [View Article][PubMed]
     [Google Scholar]
  3. Li WJ, Xu P, Tang SK, Xu LH, Kroppenstedt RM et al. Prauserella halophila sp. nov. and Prauserella alba sp. nov., moderately halophilic actinomycetes from saline soil. Int J Syst Evol Microbiol 2003; 53:1545–1549 [View Article][PubMed]
     [Google Scholar]
  4. Li Y, Tang SK, Chen YG, Wu JY, Zhi XY et al. Prauserella salsuginis sp. nov., Prauserella flava sp. nov., Prauserella aidingensis sp. nov. and Prauserella sediminis sp. nov., isolated from a salt lake. Int J Syst Evol Microbiol 2009; 59:2923–2928 [View Article][PubMed]
     [Google Scholar]
  5. Wang J, Li Y, Bian J, Tang SK, Ren B et al. Prauserella marina sp. nov., isolated from ocean sediment of the South China Sea. Int J Syst Evol Microbiol 2010; 60:985–989 [View Article][PubMed]
     [Google Scholar]
  6. Schäfer J, Martin K, Kämpfer P. Prauserella muralis sp. nov., from the indoor environment. Int J Syst Evol Microbiol 2010; 60:287–290 [View Article][PubMed]
     [Google Scholar]
  7. Liu M, Zhang L, Ren B, Yang N, Yu X et al. Prauserella shujinwangii sp. nov., a new member of the genus Prauserella isolated from Xinjiang region, China. Int J Syst Evol Microbiol 2014; 64:3833–3837 [CrossRef]
     [Google Scholar]
  8. Wu JF, Li J, You ZQ, Zhang S. Prauserella coralliicola sp. nov., isolated from the coral Galaxea fascicularis. Int J Syst Evol Microbiol 2014; 64:3341–3345 [View Article][PubMed]
     [Google Scholar]
  9. Saker R, Bouras N, Meklat A, Zitouni A, Schumann P et al. Prauserella isguenensis sp. nov., a halophilic actinomycete isolated from desert soil. Int J Syst Evol Microbiol 2015; 65:1598–1603 [View Article][PubMed]
     [Google Scholar]
  10. Almutairi A. Prauserella soli sp. nov., isolated from crude oil-contaminated soil. Int J Syst Evol Microbiol 2015; 65:3060–3065 [View Article][PubMed]
     [Google Scholar]
  11. Liu JM, Habden X, Guo L, Tuo L, Jiang ZK et al. Prauserella endophytica sp. nov., an endophytic actinobacterium isolated from Tamarix taklamakanensis. Antonie van Leeuwenhoek 2015; 107:1401–1409 [View Article][PubMed]
     [Google Scholar]
  12. Atlas RM. Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiol Rev 1981; 45:180–209[PubMed]
     [Google Scholar]
  13. Prince RG, Gramain A, Mcgenity TJ. Phydrocarbon. In Timmis KN. (editor) Handbook of Hydrocarbon and Lipid Microbiology Berlin: Springer–Verlag; 2010 pp. 1672–1692
     [Google Scholar]
  14. Bushnell LD, Haas HF. The utilization of certain hydrocarbons by microorganisms. J Bacteriol 1941; 41:653–673[PubMed]
     [Google Scholar]
  15. Reasoner DJ, Geldreich EE. A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 1985; 49:1–7[PubMed]
     [Google Scholar]
  16. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966; 16:313–340 [View Article]
     [Google Scholar]
  17. Waksman SA. The Actinomycetes. Classification, Identification and Descriptions of 268 Genera and Species vol. 2 Baltimore: Williams and Wilkins; 1961
     [Google Scholar]
  18. Kelly KL. Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors Washington, DC: US Government Print Office; 1964
     [Google Scholar]
  19. Gordon RE, Barnett DA, Handerhan JE, Pang CH-N. Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 1974; 24:54–63 [View Article]
     [Google Scholar]
  20. Williams ST, Goodfellow M, Alderson G, Wellington EM, Sneath PH et al. Numerical classification of Streptomyces and related genera. J Gen Microbiol 1983; 129:1743–1813 [View Article][PubMed]
     [Google Scholar]
  21. Pospiech A, Neumann B. A versatile quick-prep of genomic DNA from Gram-positive bacteria. Trends Genet 1995; 11:217–218 [View Article][PubMed]
     [Google Scholar]
  22. Paster BJ, Russell MK, Alpagot T, Lee AM, Boches SK et al. Bacterial diversity in necrotizing ulcerative periodontitis in HIV-positive subjects. Ann Periodontol 2002; 7:8–16 [View Article][PubMed]
     [Google Scholar]
  23. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  24. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  25. Lechevalier MP, Lechevalier H. Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 1970; 20:435–443 [View Article]
     [Google Scholar]
  26. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974; 28:226–231[PubMed]
     [Google Scholar]
  27. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  28. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
     [Google Scholar]
  29. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
     [Google Scholar]
  30. Cashion P, Holder-Franklin MA, Mccully J, Franklin M. A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 1977; 81:461–466 [View Article][PubMed]
     [Google Scholar]
  31. de Ley J, Cattoir H, Reynaerts A. The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 1970; 12:133–142 [View Article][PubMed]
     [Google Scholar]
  32. Huss VA, Festl H, Schleifer KH. Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 1983; 4:184–192 [View Article][PubMed]
     [Google Scholar]
  33. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
     [Google Scholar]
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Prauserella oleivorans sp. nov., a halophilic and thermotolerant crude-oil-degrading actinobacterium isolated from an oil-contaminated mud pit
Int J Syst Evol Microbiol 67, 3381 (2017); https://doi.org/10.1099/ijsem.0.002124
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