1887

Abstract

A crude-oil-degrading, Gram-stain-positive actinobacterial strain, RIPI, was isolated from a soil sample collected from an oil-contaminated mud pit in Khangiran oil and gas field, in the north-east of Iran. RIPI was strictly aerobic, catalase- and oxidase-positive. The strain grew with 0–12.5 % (w/v) NaCl (optimum 3–5 %), at 25–55 °C (optimum 45 °C) and at pH 6.0–9.5 (optimum pH 7.0). The results of 16S rRNA gene sequence comparative analysis indicated that RIPI represents a member of the genus Prauserella , with high phylogenetic similarity to Prauserella coralliicola SCSIO 11529 (97.5 %), Prauserella endophytica SP28S-3 (97.5 %) and Prauserella marina MS498 (97.2 %). DNA–DNA relatedness values between the novel strain and P. coralliicola DSM 45821, P. endophytica DSM 46655 and P. marina DSM 45268 were 28 , 19 and 23 %, respectively. The cell wall peptidoglycan of RIPI contained meso-diaminopimelic acid as the diamino acid and the whole-cell sugars are galactose and arabinose. The polar lipids pattern contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and two unknown phospholipids. Its cellular fatty acids pattern consisted of C17 : 1ω6c, iso-C16 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), and the major respiratory quinone was MK-9(H4). The G+C content of the genomic DNA was 69 mol%. On the basis of polyphasic taxonomic data we propose that RIPI represents a novel species of the genus Prauserella , for which the name Prauserella oleivorans sp. nov. is proposed. The type strain of Prauserella oleivorans is RIPI (=IBRC-M 10906=LMG 28389).

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2017-08-31
2019-10-16
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