@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002123, author = "Hamada, Moriyuki and Saitou, Satomi and Terahara, Takeshi and Imada, Chiaki and Tamura, Tomohiko", title = "Lysinimicrobium sediminis sp. nov., an actinobacterium isolated from estuary sediment", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "9", pages = "3393-3397", doi = "https://doi.org/10.1099/ijsem.0.002123", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002123", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "polyphasic taxonomy", keywords = "Lysinimicrobium sediminis", keywords = "Actinobacteria", keywords = "Demequinaceae", abstract = "A novel Gram-stain-positive actinobacterium, designated HT7-17T, was isolated from a sediment sample collected from the estuary of the Tama River, Japan, and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain HT7-17T was closely related to members of the genus Lysinimicrobium , with a similarity range of 97.1–98.2 %. The peptidoglycan type of strain HT7-17T was A4α, the predominant menaquinone was demethylmenaquinone DMK-9(H4) and the major fatty acids were anteiso-C15 : 0, C16 : 0 and iso-C16 : 0. The DNA G+C content was 69.9 mol%. These chemotaxonomic features corresponded to those of the genus Lysinimicrobium . Meanwhile, the differences in some phenotypic characteristics, along with the result of DNA–DNA hybridization, indicated that strain HT7-17T should be distinguished from the recognized species of the genus Lysinimicrobium . Therefore, strain HT7-17T represents a novel species of the genus Lysinimicrobium , for which the name Lysinimicrobium sediminis sp. nov. is proposed. The type strain is HT7-17T (=NBRC 112286T=TBRC 7037T).", }