1887

Abstract

A novel Gram-stain-positive actinobacterium, designated HT7-17, was isolated from a sediment sample collected from the estuary of the Tama River, Japan, and its taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain HT7-17 was closely related to members of the genus , with a similarity range of 97.1–98.2 %. The peptidoglycan type of strain HT7-17 was A4α, the predominant menaquinone was demethylmenaquinone DMK-9(H) and the major fatty acids were anteiso-C, C and iso-C. The DNA G+C content was 69.9 mol%. These chemotaxonomic features corresponded to those of the genus . Meanwhile, the differences in some phenotypic characteristics, along with the result of DNA–DNA hybridization, indicated that strain HT7-17 should be distinguished from the recognized species of the genus . Therefore, strain HT7-17 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HT7-17 (=NBRC 112286=TBRC 7037).

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/content/journal/ijsem/10.1099/ijsem.0.002123
2017-09-01
2024-03-28
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References

  1. Hamada M, Tamura T, Yamamura H, Suzuki K, Hayakawa M. Lysinimicrobium mangrovi gen. nov., sp. nov., an actinobacterium isolated from the rhizosphere of a mangrove. Int J Syst Evol Microbiol 2012; 62:1731–1735 [View Article][PubMed]
    [Google Scholar]
  2. Hamada M, Shibata C, Saitou S, Tamura T, Komaki H et al. Proposal of nine novel species of the genus Lysinimicrobium and emended description of the genus Lysinimicrobium. Int J Syst Evol Microbiol 2015; 65:4394–4402 [View Article][PubMed]
    [Google Scholar]
  3. Schleifer KH, Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 1972; 36:407–477[PubMed]
    [Google Scholar]
  4. Hamada M, Yamamura H, Komukai C, Tamura T, Suzuki K et al. Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium. J Antibiot 2012; 65:427–431 [View Article][PubMed]
    [Google Scholar]
  5. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  6. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  7. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  8. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  9. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  10. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  11. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  12. Minnikin DE, Collins MD, Goodfellow M. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 1979; 47:87–95 [View Article]
    [Google Scholar]
  13. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  14. Schumann P. Peptidoglycan sturucture. In Rainey FA, Oren A. (editors) Taxonomy of Prokaryotes vol. 38 Chennai: Academic Press; 2011 pp. 101–129 [CrossRef]
    [Google Scholar]
  15. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
    [Google Scholar]
  16. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
    [Google Scholar]
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