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Abstract

A novel Gram-stain-negative, rod-shaped, gliding bacterium, designated strain 001-Na2, was isolated from coastal sediment of the Yellow Sea in Sindu-ri, Republic of Korea. Cells were aerobic, oxidase-positive and catalase-positive. Colonies were dark salmon-coloured, circular, and pulvinate with entire margins. 16S rRNA gene sequence analysis revealed that strain 001-Na2 belonged to the genus and these species formed a distinct lineage within the family . The closest phylogenetic neighbours were ‘’ AK12 (98.1 % 16S rRNA gene sequence similarity), LMG 23754 (96.4 %), HMD 3054 (96.0 %), KMM 6172 (96.0 %) and JL 3085 (95.8 %). The predominant respiratory quinone was menaquinone MK-7. The major fatty acids were iso-C, summed feature 3 (comprising Cω7 and/or Cω6), Cω6 and summed feature 9 (comprising iso-Cω9 and/or 10-methyl C). The polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid and three unidentified lipids. The DNA G+C content was 44.6 mol%. DNA–DNA relatedness between strain 001-Na2 and ‘’ JCM 17847 was 20.8–21.1 %. On the basis of the phenotypic features, and phylogenetic and DNA–DNA hybridization analyses presented, strain 001-Na2 (=KCTC 52495=JCM 31786=DSM 103729) is the type strain of a novel species of the genus , for which the name sp. nov. is proposed.

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2017-09-01
2020-01-21
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