1887

Abstract

Seven strains representing two novel yeast species were isolated from rotting wood in Henan and Yunnan Provinces, PR China. The results of phylogenetic analysis based on the D1/D2 domains of the large subunit (LSU) rRNA gene revealed that these two species are members of the genus Kodamaea, although the formation of ascospores was not observed. Kodamaea neixiangensis f.a., sp. nov. (type strain NYNU 167139=CICC 33170=CBS 14699) formed a clade with Candida kaohsiungensis and Candida hsintzibuensis, from which it differed by 10–16 substitutions in the D1/D2 domain. The ITS sequences of K. neixiangensis sp. nov. differed by 27 substitutions from those of the type strain of C. kaohsiungensis. The most closely related species with a validly published name to Kodamaea jinghongensis f.a., sp. nov. (type strain NYNU 167162=CICC 33171=CBS 14700) was Candida fukazawae, but this differed by 14 substitutions in the D1/D2 domain and by 15 substitutions in the ITS region.

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2017-08-25
2019-12-05
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References

  1. Suh SO, Blackwell M. Four new yeasts in the Candida mesenterica clade associated with basidiocarp-feeding beetles. Mycologia 2005;97:167–177 [CrossRef][PubMed]
    [Google Scholar]
  2. Kurtzman CP, Robnett CJ. Relationships among genera of the Saccharomycotina (Ascomycota) from multigene phylogenetic analysis of type species. FEMS Yeast Res 2013;13:23–33 [CrossRef][PubMed]
    [Google Scholar]
  3. Hsieh CW, Huang LY, Tschen EF, Chang CF, Lee CF. Five novel anamorphic, ascomycetous yeast species associated with mushrooms and soil. FEMS Yeast Res 2010;10:948–956 [CrossRef][PubMed]
    [Google Scholar]
  4. Lachance MA, Kurtzman CP. Kodamaea Yamada Y, Suzuki T, Matsuda, Mikata emend. Rosa, Lachance, Starmer, Barker, Bowles and Schlag-Edler (1999). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – A Taxonomic Study, 5th ed.vol. 2 Amsterdam: Elsevier; 2011; pp.483–490[CrossRef]
    [Google Scholar]
  5. Nakase T, Jindamorakot S, Am-In S, Ninomiya S, Kawasaki H. Candida loeiensis sp. nov., a novel anamorphic yeast species found in Thailand. J Gen Appl Microbiol 2011;57:387–391 [CrossRef][PubMed]
    [Google Scholar]
  6. Freitas LF, Carvajal Barriga EJ, Barahona PP, Lachance MA, Rosa CA. Kodamaea transpacifica f.a., sp. nov., a yeast species isolated from ephemeral flowers and insects in the Galapagos Islands and Malaysia: further evidence for ancient human transpacific contacts. Int J Syst Evol Microbiol 2013;63:4324–4329 [CrossRef][PubMed]
    [Google Scholar]
  7. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP et al. Candida Berkhout (1923). In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – A Taxonomic Study, 5th ed.vol. 2 Amsterdam: Elsevier; 2011; pp.987–1278[CrossRef]
    [Google Scholar]
  8. Hui FL, Chen L, Li ZH, Niu QH, Ke T. Metschnikowia henanensis sp. nov., a new anamorphic yeast species isolated from rotten wood in China. Antonie van Leeuwenhoek 2013;103:899–904 [CrossRef][PubMed]
    [Google Scholar]
  9. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. (editors) The Yeasts – A Taxonomic Study, 5th ed.vol. 1 Amsterdam: Elsevier; 2011; pp.87–110[CrossRef]
    [Google Scholar]
  10. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998;73:331–371 [CrossRef][PubMed]
    [Google Scholar]
  11. White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. (editors) PCR Protocols: A Guide to Methods and Applications New York: Academic Press; 1990; pp.315–322
    [Google Scholar]
  12. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997;25:3389–3402 [CrossRef][PubMed]
    [Google Scholar]
  13. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  14. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  15. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980;16:111–120 [CrossRef][PubMed]
    [Google Scholar]
  16. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
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