1887

Abstract

A novel halophilic archaeon, designated strain WIIAL99, was isolated from Lake Meyghan, a hypersaline lake in Iran. Cells of strain WIIAL99 were non-motile, catalase-positive and oxidase-negative. Strain WIIAL99 required at least 2.5 M NaCl and 0.05 M MgCl for growth. Optimal growth was achieved at 3.5 M NaCl and 0.1 M MgCl. The optimum pH and temperature for growth were pH 7.0 and 37–40 °C; it was able to grow at pH 6.0–8.5 and 20–55 °C. Cells lysed in distilled water and the minimal NaCl concentration to prevent cell lysis was 8 % (w/v). The major polar lipids of strain WIIAL99 were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, disulfated diglycosyl diether and one unidentified glycolipid. The DNA G+C content of strain WIIAL99 was 66.7 mol%. The closest relative was JCM 17119 with 98.2 % similarity in the orthologous 16S rRNA gene sequence. Analysis of 16S rRNA and gene sequences indicated that strain WIIAL99 is a member of the genus in the family and forms a distinct cluster. It was concluded that strain WIIAL99 (=IBRC-M 11062=LMG 29814) represents a novel species of the genus , for which the name sp. nov. is proposed.

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2017-09-01
2020-09-30
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