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Abstract

A novel Gram-stain-positive actinobacterium, designated HIs16-32, was isolated from a sand sample collected from a mangrove tidal flat in Japan and its taxonomic position was investigated by a polyphasic approach. The cells of strain HIs16-32 were Gram-stain-positive, rod-shaped, non-motile and non-endospore-forming. Strain HIs16-32 contained glutamic acid, glycine and lysine in the peptidoglycan; however, alanine was absent. Galactose and mannose were detected as cell-wall sugars. The isoprenoid quinones were identified as MK-11, MK-12 and MK-10, and the major fatty acids as anteiso-C, anteiso-C and iso-C. The DNA G+C content was determined to be 72.2 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparison revealed that strain HIs16-32 was related to members of the family but did not form a reliable cluster with any known members of the family. The highest 16S rRNA gene sequence similarity values were obtained with species of the genera (96.1–96.8 %), (96.5–96.7 %) and (96.7 %). However, strain HIs16-32 was distinguishable from the phylogenetically related genera in terms of chemotaxonomic characteristics and phylogenetic relationship. Therefore, strain HIs16-32 is concluded to represent a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is HIs16-32 (=NBRC 112289=TBRC 7038).

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2017-09-01
2020-01-29
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