1887

Abstract

Gram-reaction-positive, strictly aerobic, motile coccoid- to rod-shaped actinobacteria, designated strains YC2-20 and YC2-19 were isolated from pieces of stalactites collected at the Yongcheon Cave in Jeju, Republic of Korea. The cells of orange-coloured colonies grew at 20–37 °C, pH 6–10 and in the absence of NaCl. In the neighbour-joining tree based on 16S rRNA gene sequences, the novel isolates formed a distant sublineage at the base of the radiation of the genus . The novel isolates shared identical 16S rRNA gene sequences to each other and revealed a 16S rRNA gene sequence similarity of 95.6 % to the closest relative, and <95 % to other members of the family . Albeit with a low similarity of 16S rRNA gene sequences and a distinct phylogenetic position, most of the chemotaxonomic characteristics were in agreement with those of the genus : -diaminopimelic acid as the diagnostic diamino acid in the cell wall; MK-9(H) as the major menaquinone; polar lipids including diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside; and a DNA G+C content of 73.1 mol%. On the basis of the phenotypic and phylogenetic distinctiveness, the novel isolates are considered to represent members of a novel species of the genus , for which the name sp. nov. is proposed, with type strain YC2-20 (=KCTC 39842=DSM 103769).

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2017-09-01
2021-07-28
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