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Volume 67, Issue 9

Description of sp. nov. and sp. nov. Free

Abstract

Three strains (JA826, JA912 and JA913), which were yellowish brown colour, rod to oval shaped, Gram-stain-negative, motile, phototrophic bacteria with a vesicular architecture of intracytoplasmic membranes, were isolated from different pond samples. The DNA G+C content of the three strains was between 64.6 and 65.5 mol%. The highest 16S rRNA gene sequence similarity of all three strains was with the type strains of the genus in the family Strain JA826 had highest sequence similarity with JA276 (98.5 %), JA737 (97.5 %) and other members of the genus (<97 %). Strain JA912 had highest sequence similarity with JA737 (99.6 %), N1 (99.3 %), ATCC 11166 (98.8 %) and less than 97 % similarity with other members of the genus . The 16S rRNA gene sequence similarity between strains JA826 and JA912 was 96.9 %. DNA–DNA hybridization showed that strains JA826 and JA912 (values among themselves and between the type strains of nearest members <44 %) did not belong to any of the nearest species of the genus . However, strains JA912 and JA913 were closely related (DNA–DNA hybridization value >90 %). The genomic distinction was also supported by differences in phenotypic and chemotaxonomic characteristics in order to propose strains JA826 (=KCTC 15478=LMG 28758) and JA912 (=KCTC 15475=LMG 28748) as new species in the genus with the names and , respectively.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): anoxygenic phototrophic bacteria and Rhodobacter, sensu stricto, sp. nov.
© 2017 IUMS
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2017-09-01
2023-12-01
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References

  1. Imhoff JF, Truper HG, Pfennig N. Rearrangement of the species and genera of the phototrophic "Purple Nonsulfur Bacteria". Int J Syst Bacteriol 1984; 34:340–343 [View Article]
     [Google Scholar]
  2. Hiraishi A, Ueda Y. Intrageneric structure of the genus Rhodobacter: transfer of Rhodobacter sulfidophilus and related marine species to the genus Rhodovulum gen. nov. Int J Syst Bacteriol 1994; 44:15–23 [View Article]
     [Google Scholar]
  3. Subhash Y, Lee SS. Rhodobacter sediminis sp. nov., isolated from lagoon sediments. Int J Syst Evol Microbiol 2016; 66:2965–2970 [View Article][PubMed]
     [Google Scholar]
  4. Archana A, Sasikala C, Ramana C, Arunasri K. "Paraffin wax-overlay of pour plate", a method for the isolation and enumeration of purple non-sulfur bacteria. J Microbiol Methods 2004; 59:423–425 [View Article][PubMed]
     [Google Scholar]
  5. Lakshmi KV, Sasikala C, Takaichi S, Ramana C. Phaeospirillum oryzae sp. nov., a spheroplast-forming, phototrophic alphaproteobacterium from a paddy soil. Int J Syst Evol Microbiol 2011; 61:1656–1661 [View Article][PubMed]
     [Google Scholar]
  6. Srinivas A, Vinay Kumar B, Divya Sree B, Tushar L, Sasikala C et al. Rhodovulum salis sp. nov. and Rhodovulum viride sp. nov., phototrophic Alphaproteobacteria isolated from marine habitats. Int J Syst Evol Microbiol 2014; 64:957–962 [View Article][PubMed]
     [Google Scholar]
  7. Hanada S, Takaichi S, Matsuura K, Nakamura K. Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. Int J Syst Evol Microbiol 2002; 52:187–193 [View Article][PubMed]
     [Google Scholar]
  8. Sasikala K, Ramana CV, Raghuveer Rao P, Subrahmanyam M. Photoproduction of hydrogen, nitrogenase and hydrogenase activities of free and immobilized whole cells of Rhodobacter sphaeroides O.U. 001. FEMS Microbiol Lett 1990; 72:23–28 [View Article]
     [Google Scholar]
  9. Trüper HG, Pfennig N. Characterization and identification of the anoxygenic phototrophic bacteria. In Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG et al. (editors) The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria vol. 1 New York: Springer; 1981 pp. 298–312
     [Google Scholar]
  10. Divyasree B, Lakshmi KV, Bharti D, Sasikala C, Ramana C. Rhodovulum aestuarii sp. nov., isolated from a brackish water body. Int J Syst Evol Microbiol 2016; 66:165–171 [View Article][PubMed]
     [Google Scholar]
  11. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the g+c content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
     [Google Scholar]
  12. Subhash Y, Sasikala C, Ramana C. Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. Int J Syst Evol Microbiol 2013; 63:3463–3469 [View Article][PubMed]
     [Google Scholar]
  13. Subhash Y, Tushar L, Sasikala C, Ramana C. Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. Int J Syst Evol Microbiol 2013; 63:4524–4532 [View Article][PubMed]
     [Google Scholar]
  14. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: [View Article][PubMed]
     [Google Scholar]
  15. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  16. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article][PubMed]
     [Google Scholar]
  17. Tourova T, Antonov AS. Identification of microorganisms by rapid DNA-DNA hybridization. Methods Microbiol 1987; 19:333–355 [CrossRef]
     [Google Scholar]
  18. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Newark, DE: MIDI Inc; 1990
     [Google Scholar]
  19. Kates M. Techniques of lipidology: isolation, analysis and identification of lipids. In Burdon RH, van Knippenberg PH. (editors) Laboratory Techniques in Biochemistry and Molecular Biology, Part 2 vol. 3 Amsterdam: Elsevier; 1986 pp. 100–112
     [Google Scholar]
  20. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990; 66:199–202 [View Article]
     [Google Scholar]
  21. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13:128–130 [View Article]
     [Google Scholar]
  22. Oren A, Duker S, Ritter S. The polar lipid composition of walsby's square bacterium. FEMS Microbiol Lett 1996; 138:135–140 [View Article]
     [Google Scholar]
  23. Kates M. Techniques in Lipidology. In Work TS, Work E. (editors) Laboratory Techniques in Biochemistry and Molecular Biology , vol. 3 New York: American Elsevier Publishing Company; 1972 pp. 355–356
     [Google Scholar]
  24. Imhoff. Quinones of phototrophic purple bacteria. FEMS Microbiol Lett 1994; 25:85–89 [CrossRef]
     [Google Scholar]
  25. Hiraishi A, Hoshino Y. Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. J Gen Appl Microbiol 1984; 30:435–448 [View Article]
     [Google Scholar]
  26. Hiraishi A, Hoshino Y, Kitamura H. Isoprenoid quinone composition in the classification of Rhodospirillaceae. J Gen Appl Microbiol 1984; 30:197–210 [View Article]
     [Google Scholar]
  27. Lodha TD, Srinivas A, Sasikala C, Ramana CV. Hopanoid inventory of Rhodoplanes spp. Arch Microbiol 2015; 197:861–867 [View Article][PubMed]
     [Google Scholar]
  28. Stackebrandt E, Goebel BM. Taxonomic note: a place for dna-dna reassociation and 16s rrna sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
     [Google Scholar]
  29. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
     [Google Scholar]
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Description of Rhodobacter azollae sp. nov. and Rhodobacter lacus sp. nov.
Int J Syst Evol Microbiol 67, 3289 (2017); https://doi.org/10.1099/ijsem.0.002107
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