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Abstract

Three strains (JA826, JA912 and JA913), which were yellowish brown colour, rod to oval shaped, Gram-stain-negative, motile, phototrophic bacteria with a vesicular architecture of intracytoplasmic membranes, were isolated from different pond samples. The DNA G+C content of the three strains was between 64.6 and 65.5 mol%. The highest 16S rRNA gene sequence similarity of all three strains was with the type strains of the genus Rhodobacter sensu stricto in the family Rhodobacteraceae . Strain JA826 had highest sequence similarity with Rhodobacter maris JA276 (98.5 %), Rhodobacter viridis JA737 (97.5 %) and other members of the genus Rhodobacter (<97 %). Strain JA912 had highest sequence similarity with Rhodobacter viridis JA737 (99.6 %), Rhodobacter sediminis N1 (99.3 %), Rhodobacter capsulatus ATCC 11166 (98.8 %) and less than 97 % similarity with other members of the genus Rhodobacter . The 16S rRNA gene sequence similarity between strains JA826 and JA912 was 96.9 %. DNA–DNA hybridization showed that strains JA826 and JA912 (values among themselves and between the type strains of nearest members <44 %) did not belong to any of the nearest species of the genus Rhodobacter . However, strains JA912 and JA913 were closely related (DNA–DNA hybridization value >90 %). The genomic distinction was also supported by differences in phenotypic and chemotaxonomic characteristics in order to propose strains JA826 (=KCTC 15478=LMG 28758) and JA912 (=KCTC 15475=LMG 28748) as new species in the genus Rhodobacter sensu stricto with the names Rhodobacter lacus and Rhodobacter azollae, respectively.

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2017-08-22
2019-10-14
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