1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 67, Issue 8

sp. nov., isolated from the rhizosphere of a tomato plant Free

Abstract

A yellow, aerobic, Gram-stain-negative, non-motile, rod-shaped and non-flagellated bacterial strain, designated T16R-129, was isolated from the rhizosphere of a tomato plant collected at a farm located on Buyeo-gun of Chungcheongnam-do, South Korea. Strain T16R-129 grew at 15–40 °C and pH 7.0–9.0, and did not require NaCl for growth. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T16R-129 clustered with members of the genus , and it shared highest similarity with R9-86 (96.1 %), QH (95.9 %), DY (94.9 %), M1-33108 (95.4 %) and CR94 (95.3 %). The major isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were iso-C, iso-C 3-OH and iso-C G. The polar lipids of strain T16R-129 were phosphatidylethanolamine, two unidentified aminolipids, two unidentified aminophospholipids and five unidentified polar lipids. The G+C content of the genomic DNA was 46.0 mol%. On the basis of data from this polyphasic taxonomic study, strain T16R-129 represents a novel species in the genus , for which the name sp. nov. is proposed; the type strain is T16R-129 (=KACC 18787=JCM 31603).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene , polar lipid , quinone and Terrimonas terrae
© 2017 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002104
2017-08-01
2023-06-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/8/3105.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002104&mimeType=html&fmt=ahah

References

  1. Zhang J, Gu T, Zhou Y, He J, Zheng LQ et al. Terrimonas rubra sp. nov., isolated from a polluted farmland soil and emended description of the genus Terrimonas. Int J Syst Evol Microbiol 2012; 62:2593–2597 [View Article][PubMed]
     [Google Scholar]
  2. Jin D, Wang P, Bai Z, Jin B, Yu Z et al. Terrimonas pekingensis sp. nov., isolated from bulking sludge, and emended descriptions of the genus Terrimonas, Terrimonas ferruginea, Terrimonas lutea and Terrimonas aquatica. Int J Syst Evol Microbiol 2013; 63:1658–1664 [View Article][PubMed]
     [Google Scholar]
  3. Han SI, Lee YR, Kim JO, Whang KS. Terrimonas rhizosphaerae sp. nov., isolated from ginseng rhizosphere soil. Int J Syst Evol Microbiol 2017; 67:391–395 [View Article][PubMed]
     [Google Scholar]
  4. Kim MC, Kang OC, Kim CM, Zhang Y, Liu Z et al. Terrimonas crocea sp nov., isolated from the till of high Arctic glacier Midtre Lovénbreen in the West Svalbard Archipelago. Int J Syst Evol Microbiol 2017; 67:868–874 [CrossRef]
     [Google Scholar]
  5. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
     [Google Scholar]
  6. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
     [Google Scholar]
  7. Pruesse E, Peplies J, Glöckner FO. SINA: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics 2012; 28:1823–1829 [View Article][PubMed]
     [Google Scholar]
  8. Kimura M. The Neutral Theory of Molecular Evolution Cambridge: Cambridge University Press; 1983 [CrossRef]
     [Google Scholar]
  9. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  10. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
     [Google Scholar]
  11. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
     [Google Scholar]
  12. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
     [Google Scholar]
  13. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  14. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
     [Google Scholar]
  15. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI; 1990
     [Google Scholar]
  17. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  18. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4:770–773 [View Article][PubMed]
     [Google Scholar]
  19. Jiang F, Qiu X, Chang X, Qu Z, Ren L et al. Terrimonas arctica sp. nov., isolated from Arctic tundra soil. Int J Syst Evol Microbiol 2014; 64:3798–3803 [View Article][PubMed]
     [Google Scholar]
  20. Sheu SY, Cho NT, Arun AB, Chen WM. Terrimonas aquatica sp. nov., isolated from a freshwater spring. Int J Syst Evol Microbiol 2010; 60:2705–2709 [View Article][PubMed]
     [Google Scholar]
  21. Xie CH, Yokota A. Reclassification of [Flavobacterium] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. Int J Syst Evol Microbiol 2006; 56:1117–1121 [View Article][PubMed]
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002104
Loading
Terrimonas terrae sp. nov., isolated from the rhizosphere of a tomato plant
Int J Syst Evol Microbiol 67, 3105 (2017); https://doi.org/10.1099/ijsem.0.002104
/content/journal/ijsem/10.1099/ijsem.0.002104
/content/journal/ijsem/10.1099/ijsem.0.002104
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error