1887

Abstract

A Gram-stain-negative, aerobic, rod-shaped and motile bacterium, designated M23, was isolated from a laboratory culture of a bloom-forming cyanobacterium, , which was isolated from a eutrophic lake in Korea. The strain grew optimally without NaCl and at 25–30 °C on R2A agar medium. Phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the genus , with the highest similarity to DSM 16282 (98.5 %). DNA–DNA relatedness between strain M23 and the closely related species in the genus was <30 %. Strain M23 contained iso-C, summed feature 3 (C 7 and/or C 6) and iso-C as major fatty acids and ubiquinone-8 (Q-8) as the major quinone. Strain M23 contained diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylmethylethanolamine as major polar lipids. The DNA G+C content of strain M23 was 69.6 mol%. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain M23 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is M23 (=KCTC 52219=JCM 31889)

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2017-09-01
2024-03-29
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References

  1. Graham JL, Loftin KA, Meyer MT, Ziegler AC. Cyanotoxin mixtures and taste-and-odor compounds in cyanobacterial blooms from the Midwestern United States. Environ Sci Technol 2010; 44:7361–7368 [View Article][PubMed]
    [Google Scholar]
  2. Smith JL, Boyer GL, Zimba PV. A review of cyanobacterial odorous and bioactive metabolites: impacts and management alternatives in aquaculture. Aquaculture 2008; 280:5–20 [View Article]
    [Google Scholar]
  3. Srivastava A, Singh S, Ahn CY, Oh HM, Asthana RK. Monitoring approaches for a toxic cyanobacterial bloom. Environ Sci Technol 2013; 47:8999–9013 [View Article][PubMed]
    [Google Scholar]
  4. Cole JJ. Interactions between bacteria and algae in aquatic ecosystems. Annu Rev Ecol Syst 1982; 13:291–314 [View Article]
    [Google Scholar]
  5. Eigemann F, Hilt S, Salka I, Grossart HP. Bacterial community composition associated with freshwater algae: species specificity vs. dependency on environmental conditions and source community. FEMS Microbiol Ecol 2013; 83:650–663 [View Article][PubMed]
    [Google Scholar]
  6. Ramanan R, Kang Z, Kim BH, Cho DH, Jin L et al. Phycosphere bacterial diversity in green algae reveals an apparent similarity across habitats. Algal Res 2015; 8:140–144 [View Article]
    [Google Scholar]
  7. Bagatini IL, Eiler A, Bertilsson S, Klaveness D, Tessarolli LP et al. Host-specificity and dynamics in bacterial communities associated with bloom-forming freshwater phytoplankton. PLoS ONE 2014; 9:e85950 [View Article][PubMed]
    [Google Scholar]
  8. Lee EM, Jeon CO, Choi I, Chang KS, Kim CJ. Silanimonas lenta gen. nov., sp. nov., a slightly thermophilic and alkaliphilic gammaproteobacterium isolated from a hot spring. Int J Syst Evol Microbiol 2005; 55:385–389 [View Article][PubMed]
    [Google Scholar]
  9. Srinivas TN, Kailash TB, Anil Kumar P. Silanimonas mangrovi sp. nov., a member of the family Xanthomonadaceae isolated from mangrove sediment, and emended description of the genus Silanimonas. Int J Syst Evol Microbiol 2013; 63:274–279 [View Article][PubMed]
    [Google Scholar]
  10. Dastager SG, Mawlankar R, Mual P, Verma A, Krishnamurthi S et al. Bacillus encimensis sp. nov. isolated from marine sediment. Int J Syst Evol Microbiol 2015; 65:1421–1425 [View Article][PubMed]
    [Google Scholar]
  11. Tarrand JJ, Gröschel DH, Rapid GDH. Rapid, modified oxidase test for oxidase-variable bacterial isolates. J Clin Microbiol 1982; 16:772–774[PubMed]
    [Google Scholar]
  12. Jin L, Lee HG, La HJ, Ko SR, Ahn CY et al. Ferruginibacter profundus sp. nov., a novel member of the family Chitinophagaceae, isolated from freshwater sediment of a reservoir. Antonie Van Leeuwenhoek 2014; 106:319–323 [View Article][PubMed]
    [Google Scholar]
  13. Lane D. 16S/23S rRNA Sequencing. In: Nucleic Acid Techniques in Bacterial Systematics Chichester, UK: John Wiley and Sons; 1991 pp. 115–175
    [Google Scholar]
  14. Jin L, Ko SR, Cui Y, Lee CS, Oh HM et al. Pusillimonas caeni sp. nov., isolated from a sludge sample of a biofilm reactor. Antonie van Leeuwenhoek 2017; 110:125–132 [View Article][PubMed]
    [Google Scholar]
  15. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
    [Google Scholar]
  16. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  17. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  18. Kimura M. The Neutral Theory of Molecular Evolution Cambridge University Press; 1984
    [Google Scholar]
  19. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  20. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  21. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  22. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  23. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  24. Tamaoka J, Komagata K. Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 1984; 25:125–128 [View Article]
    [Google Scholar]
  25. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [View Article]
    [Google Scholar]
  26. Cui YS, Lee JS, Lee ST, Im WT. Kribbella ginsengisoli sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2010; 60:364–368 [View Article][PubMed]
    [Google Scholar]
  27. Cui Y, Baek SH, Wang L, Lee HG, Cui C et al. Streptomyces panacagri sp. nov., isolated from soil of a ginseng field. Int J Syst Evol Microbiol 2012; 62:780–785 [View Article][PubMed]
    [Google Scholar]
  28. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  29. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B et al. Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 1997; 47:1129–1133 [View Article][PubMed]
    [Google Scholar]
  30. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977; 27:104–117 [View Article]
    [Google Scholar]
  31. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [View Article]
    [Google Scholar]
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