1887

Abstract

A Gram-stain-negative, non-flagellated, gliding, non-spore-forming bacterial strain, designated RA3-2, was isolated from the gut of an abalone (Haliotis discus hannai) collected from the sea around Jeju island, South Korea, and subjected to a polyphasic taxonomic study. RA3-2 grew optimally at 20 °C and in the presence of 2.0–3.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences indicated that RA3-2 fell within the clade comprising the type strains of species of the genus Tenacibaculum , clustering with the type strains of Tenacibaculum soleae , Tenacibaculum ovolyticum and Tenacibaculum dicentrarchi ; showing 16S rRNA gene sequence similarity values of 96.2–96.8 %. The novel strain exhibited 16S rRNA gene sequence similarity values of 93.5–96.9 % to the type strains of the other species of the genus Tenacibaculum . RA3-2 contained MK-6 as the predominant menaquinone and iso-C15 : 0 and iso-C15 : 0 3-OH as the major fatty acids. The major polar lipids of RA3-2 were phosphatidylethanolamine, two unidentified lipids, one unidentified aminophospholipid and one unidentified glycolipid. The DNA G+C content of RA3-2 was 31.7 mol%. The differential phenotypic properties, together with the phylogenetic data, revealed that RA3-2 is separated from other species of the genus Tenacibaculum with validly published names. On the basis of the data presented, RA3-2 is considered to represent a novel species of the genus Tenacibaculum , for which the name Tenacibaculum haliotis sp. nov. is proposed. The type strain is RA3-2 (=KCTC 52419=NBRC 112382).

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2017-08-22
2019-10-20
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