1887

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Volume 67, Issue 9

gen. nov., sp. nov., a marine bacterium of the family isolated from coastal seawater Free

Abstract

A Gram-stain-negative, aerobic, yellow-pigmented, straight rod-shaped bacterium, strain MOLA117, was isolated from a coastal water sample from the north-western Mediterranean Sea, near Banyuls-sur-Mer, France. On the basis of phylogenetic analysis of the 16S rRNA gene sequence, strain MOLA117 was placed within the family , but showed less than 93 % 16S rRNA gene sequence similarity to other recognized species within the family. The most closely related genera included , , and . The only isoprenoid quinone was menaquinone MK-6 and the predominant fatty acid was iso-C 3-OH, representing over 33 % of the total fatty acids. The DNA G+C content was 36.9 mol%. Strain MOLA117 required NaCl for growth, and did not exhibit gliding motility or produce flexirubin. Based on the phenotypic and phylogenetic data, strain MOLA117 should be considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is MOLA117 (=CIP 110873=DSM 29762).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Flavobacteriaceae and marine coastal waters
© 2017 IUMS
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2017-09-01
2024-04-19
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References

  1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990; 215:403–410 [View Article][PubMed]
     [Google Scholar]
  2. Zbinden M, Pailleret M, Ravaux J, Gaudron SM, Hoyoux C et al. Bacterial communities associated with the wood-feeding gastropod Pectinodonta sp. (Patellogastropoda, Mollusca). FEMS Microbiol Ecol 2010; 74:450–463 [View Article][PubMed]
     [Google Scholar]
  3. Fagervold SK, Urios L, Intertaglia L, Batailler N, Lebaron P et al. Pleionea mediterranea gen. nov., sp. nov., a gammaproteobacterium isolated from coastal seawater. Int J Syst Evol Microbiol 2013; 63:2700–2705 [View Article][PubMed]
     [Google Scholar]
  4. Raguénès G, Christen R, Guezennec J, Pignet P, Barbier G. Vibrio diabolicus sp. nov., a new polysaccharide-secreting organism isolated from a deep-sea hydrothermal vent polychaete annelid, Alvinella pompejana. Int J Syst Bacteriol 1997; 47:989–995 [View Article][PubMed]
     [Google Scholar]
  5. Powers EM. Efficacy of the ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 1995; 61:3756–3758[PubMed]
     [Google Scholar]
  6. Lányi B. 1 classical and rapid identification methods for medically important bacteria. Curr Methods Classif Identif Microorg 1988; 19:1–67
     [Google Scholar]
  7. Bruns A, Rohde M, Berthe-Corti L. Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 2001; 51:1997–2006 [View Article][PubMed]
     [Google Scholar]
  8. Smibert RM, Krieg NR. General characterization. In Manuals of Methods for General Bacteriology Washington, DC: American Society for Microbiology; 1981 pp. 409–443
     [Google Scholar]
  9. Barbeyron T, L'Haridon S, Corre E, Kloareg B, Potin P. Zobellia galactanovorans gen. nov., sp. nov., a marine species of Flavobacteriaceae isolated from a red alga, and classification of [Cytophaga] uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Zobellia uliginosa gen. nov., comb. nov. Int J Syst Evol Microbiol 2001; 51:985–997 [View Article][PubMed]
     [Google Scholar]
  10. Eiler A, Bertilsson S. Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes. Environ Microbiol 2004; 6:1228–1243 [View Article][PubMed]
     [Google Scholar]
  11. Bell PJ, Sunna A, Gibbs MD, Curach NC, Nevalainen H et al. Prospecting for novel lipase genes using PCR. Microbiology 2002; 148:2283–2291 [View Article][PubMed]
     [Google Scholar]
  12. Acinas SG, Sarma-Rupavtarm R, Klepac-Ceraj V, Polz MF. PCR-induced sequence artifacts and bias: insights from comparison of two 16S rRNA clone libraries constructed from the same sample. Appl Environ Microbiol 2005; 71:8966–8969 [View Article][PubMed]
     [Google Scholar]
  13. Ludwig W, Strunk O, Westram R, Richter L, Meier H et al. ARB: a software environment for sequence data. Nucleic Acids Res 2004; 32:1363–1371 [View Article][PubMed]
     [Google Scholar]
  14. Stamatakis A, Hoover P, Rougemont J. A rapid bootstrap algorithm for the RAxML Web servers. Syst Biol 2008; 57:758–771 [View Article][PubMed]
     [Google Scholar]
  15. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article][PubMed]
     [Google Scholar]
  16. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50:1861–1868 [View Article][PubMed]
     [Google Scholar]
  17. Barbeyron T, Carpentier F, L'Haridon S, Schüler M, Michel G et al. Description of Maribacter forsetii sp. nov., a marine Flavobacteriaceae isolated from North Sea water, and emended description of the genus Maribacter. Int J Syst Evol Microbiol 2008; 58:790–797 [View Article][PubMed]
     [Google Scholar]
  18. Ivanova EP, Nedashkovskaya OI, Chun J, Lysenko AM, Frolova GM et al. Arenibacter gen. nov., new genus of the family Flavobacteriaceae and description of a new species, Arenibacter latericius sp. nov. Int J Syst Evol Microbiol 2001; 51:1987–1995 [View Article][PubMed]
     [Google Scholar]
  19. Nedashkovskaya OI, Vancanneyt M, Cleenwerck I, Snauwaert C, Kim SB et al. Arenibacter palladensis sp. nov., a novel marine bacterium isolated from the green alga Ulva fenestrata, and emended description of the genus Arenibacter. Int J Syst Evol Microbiol 2006; 56:155–160 [View Article][PubMed]
     [Google Scholar]
  20. Park S, Oh KH, Lee SY, Oh TK, Yoon JH. Cellulophaga geojensis sp. nov., a member of the family Flavobacteriaceae isolated from marine sand. Int J Syst Evol Microbiol 2012; 62:1354–1358 [View Article][PubMed]
     [Google Scholar]
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Saonia flava gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from coastal seawater
Int J Syst Evol Microbiol 67, 3246 (2017); https://doi.org/10.1099/ijsem.0.002095
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