RT Journal Article SR Electronic(1) A1 Lu, De-Chen A1 Xia, Jun A1 Dunlap, Christopher A. A1 Rooney, Alejandro P. A1 Du, Zong-JunYR 2017 T1 Gracilimonas halophila sp. nov., isolated from a marine solar saltern JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 9 SP 3251 OP 3255 DO https://doi.org/10.1099/ijsem.0.002093 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative and facultatively anaerobic bacterium, designated WDS2C40T, was isolated from a marine solar saltern in Weihai, China. Cells of strain WDS2C40T were 0.4–0.5 µm wide and 4.0–9.0 µm long, catalase-positive and oxidase-negative. Strain WDS2C40T was tolerant to moderate salt concentrations. Growth occurred at 20–42 °C (optimum, 37–40 °C), at pH 7.0–8.5 (optimum, 7.5–8.0) and with 2–16 % (w/v) NaCl (optimum, 6–8 %). A phylogenetic analysis of the 16S rRNA gene indicated that strain WDS2C40T was a member of the genus Gracilimonas within the family Balneolaceae . The most closely related neighbour was Gracilimonas rosea JCM 18898T (95.92 % similarity). The major respiratory quinone of strain WDS2C40T was menaquinone MK-7, and the dominant fatty acids were iso-C13 : 0, iso-C15 : 0 and summed feature 3. The major polar lipids were diphosphatidylglycerol, one kind of glycolipid and two unidentified phospholipids. The genomic DNA G+C content was 41.7 mol%. Based on this polyphasic taxonomic study, strain WDS2C40T is considered to represent a novel species in the genus Gracilimonas , for which the name Gracilimonas halophila sp. nov. is proposed. The type strain is WDS2C40T (=KCTC 52042T=MCCC 1H00135T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002093