1887

Abstract

A Gram-stain-negative and facultatively anaerobic bacterium, designated WDS2C40, was isolated from a marine solar saltern in Weihai, China. Cells of strain WDS2C40 were 0.4–0.5 µm wide and 4.0–9.0 µm long, catalase-positive and oxidase-negative. Strain WDS2C40 was tolerant to moderate salt concentrations. Growth occurred at 20–42 °C (optimum, 37–40 °C), at pH 7.0–8.5 (optimum, 7.5–8.0) and with 2–16 % (w/v) NaCl (optimum, 6–8 %). A phylogenetic analysis of the 16S rRNA gene indicated that strain WDS2C40 was a member of the genus Gracilimonas within the family Balneolaceae . The most closely related neighbour was Gracilimonas rosea JCM 18898 (95.92 % similarity). The major respiratory quinone of strain WDS2C40 was menaquinone MK-7, and the dominant fatty acids were iso-C13 : 0, iso-C15 : 0 and summed feature 3. The major polar lipids were diphosphatidylglycerol, one kind of glycolipid and two unidentified phospholipids. The genomic DNA G+C content was 41.7 mol%. Based on this polyphasic taxonomic study, strain WDS2C40 is considered to represent a novel species in the genus Gracilimonas , for which the name Gracilimonas halophila sp. nov. is proposed. The type strain is WDS2C40 (=KCTC 52042=MCCC 1H00135).

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2017-08-22
2019-10-21
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