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Abstract

A novel actinomycete, designated strain EURKPP3H10, was isolated from surface-sterilized roots of Eucalyptus camaldulensis Dehnh., collected from Kamphaengphet Silvicultural Research Station, Kamphaengphet province, Thailand. The taxonomic position of strain EURKPP3H10 was studied using a polyphasic approach. Phylogenetic evaluation based on 16S rRNA gene sequence analysis showed that strain EURKPP3H10 belongs to the genus Cryptosporangium , with the highest sequence similarity to Cryptosporangium cibodasense LIPI11-2-Ac046 (99.2 %). Colonies of strain EURKPP3H10 were orange yellow. Spherical sporangia with motile spores were observed. The strain contained meso-diaminopimelic acid and acofriose, arabinose, galactose, glucose, mannose, xylose and ribose in whole-cell hydrolysates. The predominant menaquinones were MK-9(H8) and MK-9(H6). The major fatty acids were iso-C16 : 0, C17 : 1ω8c, C18 : 1ω9c and C17 : 0. The polar lipids of the strain were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and unknown lipids. The DNA G+C content of the genomic DNA was 71.5 mol%. Based on comparative analysis of physiological, biochemical and chemotaxonomic data, including DNA–DNA hybridization, strain EURKPP3H10 represents a novel species of the genus Cryptosporangium , for which the name Cryptosporangium eucalypti sp. nov. is proposed. The type strain is EURKPP3H10 (=BCC 77605=NBRC 111482).

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2017-08-31
2019-10-22
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