1887

Abstract

A Gram-positive, strictly aerobic, non-sporulating, motile, rod-shaped bacterium, designated YC2-25, was isolated from pieces of stalactites collected from a lava cave in Jeju, Republic of Korea. Colonies were circular, smooth, convex and cream to light yellow in colour. A neighbour-joining tree based on 16S rRNA gene sequences indicated that the organism belonged to the genus Conexibacter with the closest relatives being Conexibacter woesei (99.0 % sequence similarity) and Conexibacter arvalis (98.8 %). Meso-Diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The predominant menaquinone was MK-7(H4). The polar lipids were diphosphatidylglycerol, phosphatidylinositol, two phospholipids and four lipids. The predominant fatty acid was C18 : 1ω9c. The DNA G+C content was 70.3 %. On the basis of distant phenotypic characteristics and DNA–DNA hybridization data, strain YC2-25 (=KCTC 39840=DSM 103719) represents a novel species of the genus Conexibacter , for which the name Conexibacter stalactiti sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002083
2017-09-04
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/9/3214.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002083&mimeType=html&fmt=ahah

References

  1. Monciardini P, Cavaletti L, Schumann P, Rohde M, Donadio S. Conexibacter woesei gen. nov., sp. nov., a novel representative of a deep evolutionary line of descent within the class Actinobacteria. Int J Syst Evol Microbiol 2003;53:569–576 [CrossRef][PubMed]
    [Google Scholar]
  2. Seki T, Matsumoto A, Shimada R, Inahashi Y, Omura S et al. Conexibacter arvalis sp. nov., isolated from a cultivated field soil sample. Int J Syst Evol Microbiol 2012;62:2400–2404 [CrossRef][PubMed]
    [Google Scholar]
  3. Shirling EB, Gottlieb D. Methods for characterization of Streptomyces species. Int J Syst Bacteriol 1966;16:313–340 [CrossRef]
    [Google Scholar]
  4. Ko KS, Kim Y, Seong CN, Lee SD. Rhodococcus antrifimi sp. nov., isolated from dried bat dung of a cave. Int J Syst Evol Microbiol 2015;65:4043–4048 [CrossRef][PubMed]
    [Google Scholar]
  5. Collins MD. Analysis of isoprenoid quinones. Methods Microbiol 1985;18:329–366[CrossRef]
    [Google Scholar]
  6. Kroppenstedt RM. Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985; pp.173–199
    [Google Scholar]
  7. Minnikin DE, Patel PV, Alshamaony L, Goodfellow M. Polar lipid composition in the classification of Nocardia and related bacteria. Int J Syst Bacteriol 1977;27:104–117 [CrossRef]
    [Google Scholar]
  8. Minnikin DE, Hutchinson IG, Caldicott AB, Goodfellow M. Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 1980;188:221–233 [CrossRef]
    [Google Scholar]
  9. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974;28:226–231[PubMed]
    [Google Scholar]
  10. Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ et al. Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich: John Innes Foundation; 1985
    [Google Scholar]
  11. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989;39:159–167 [CrossRef]
    [Google Scholar]
  12. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989;39:224–229 [CrossRef]
    [Google Scholar]
  13. Kim OS, Cho YJ, Lee K, Yoon SH, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012;62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  14. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997;25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  15. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987;4:406–425[PubMed]
    [Google Scholar]
  16. Jukes TH, Cantor CR. Evolution of protein molecules. In Munro HN. (editor) Mammalian Protein Metabolism New York: Academic Press; 1969; pp.21–132[CrossRef]
    [Google Scholar]
  17. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985;39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  18. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981;17:368–376 [CrossRef][PubMed]
    [Google Scholar]
  19. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971;20:406–416 [CrossRef]
    [Google Scholar]
  20. Reddy GS, Garcia-Pichel F. Description of Patulibacter americanus sp. nov., isolated from biological soil crusts, emended description of the genus Patulibacter Takahashi et al. 2006 and proposal of Solirubrobacterales ord. nov. and Thermoleophilales ord. nov. Int J Syst Evol Microbiol 2009;59:87–94 [CrossRef][PubMed]
    [Google Scholar]
  21. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 1987;37:463–464 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002083
Loading
/content/journal/ijsem/10.1099/ijsem.0.002083
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error