sp. nov., isolated from abalone species Free

Abstract

A Gram-negative, aerobic, polar-flagellated and rod-shaped, sometimes slightly curved bacterium, designated MA5, was isolated from the gut of an abalone of the species collected in Japan. Phylogenetic analyses based on 16S rRNA, , and gene sequences placed strain MA5 in the genus in an independent phylogenetic line. Comparison of the 16S rRNA gene sequence of this strain with those of the type strains of the established species revealed (95.1 %) as nearest neighbour. Strain MA5 grew optimally at 25 °C, pH 6.0 to 9.0 and in the presence of 2 to 5 % (w/v) NaCl under both aerobic and microaerobic conditions. The predominant fatty acids found were summed feature 3 (iso-C 2-OH and/or C ω7), C 3-OH and C ω7. Menaquinone-6 (MK-6) and menaquinone-7 (MK-7) were found as the major respiratory quinones. The major polar lipids detected were phosphatidylethanolamine and phosphatidylglycerol. Strain MA5 could be differentiated phenotypically from the phylogenetic closest species by its ability to grow on 0.05 % safranin and 0.01 % 2,3,5-triphenyl tetrazolium chloride (TTC), but not on 0.5 % NaCl. The obtained DNA G+C content of strain MA5 was 27.9 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic distinctiveness of MA5, this strain is considered to represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is MA5 (=LMG 28652=JCM 31147).

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2017-08-01
2024-03-28
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