1887

Abstract

Two Gram-negative, rod-shaped, motile bacterial strains, named CPA5 and BR75, were isolated from the halophyte . Both presented optimum growth at 30 °C, pH 7.0–7.5 and 1–2 % NaCl (w/v) for strain CPA5, and pH 7.5–8.0 and 2 % NaCl (w/v) for strain BR75. Phylogenetic analyses based on 16S rRNA gene sequences affiliated both strains to the genus . CPA5 presented highest 16S rRNA gene sequence similarity with KYW147 (96.5 %), followed by KYW48 (95.9 %), H25 (95.6 %) and Y2 (95.5 %). BR75 displayed highest similarity with MSW-14 (96.5 %), followed by S3-63, SW-109 and MSSRF26 (96.1 %). Neither strain contained Bacteriochlorophyll . The main fatty acids observed for CPA5 were Cω6 and summed features 3 (Cω7 and/or iso-C 2-OH) and 8 (C ω7 and/or Cω6). The latter summed feature was the dominant fatty acid observed for strain BR75 as well. The major polar lipids were phosphatidylethanolamine, unidentified phospholipids and unidentified glycolipids for both strains. The predominant ubiquinone was Q-10 for both strains, and the DNA G+C contents were 63.4 mol% and 58.3 mol% for CPA5 and BR75, respectively. Based on phenotypic and genotypic results, both strains represent novel species belonging to the genus for which the names sp. nov. (type strain CPA5=CECT 9130=LMG 29519) and sp. nov (type strain BR75=CECT 9129=LMG 29518) are proposed.

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2017-08-01
2024-12-10
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