@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.002073, author = "Lee, Soon Dong", title = "Pigmentiphaga aceris sp. nov., isolated from tree sap", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2017", volume = "67", number = "9", pages = "3198-3202", doi = "https://doi.org/10.1099/ijsem.0.002073", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002073", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "new taxa, proteobacteria", keywords = "16S rRNA gene", keywords = "polyphasic investigation", keywords = "Pigmentiphaga aceris sp. nov.", abstract = "Two Gram-stain-negative bacterial strains, SAP-32T and SAP-36, were isolated from sap drawn from the Acer pictum from Mount Halla in Jeju, Republic of Korea. The organisms were strictly aerobic, non-sporulating, motile rods and showed growth at 10–30 °C, pH 7–8 and with 0–2 % NaCl. The major isoprenoid quinone was Q-8. The predominant fatty acids were C16 : 0, cyclo-C17 : 0, summed feature 3 and C18 : 0. The polar lipids contained phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unknown aminophosphoglycolipid, an unknown glycolipid, an unknown phospholipid and two unknown lipids. The DNA G+C content was 64.4 mol%. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that SAP-32T and SAP-36 formed a distinct cluster with members of the genus Pigmentiphaga within the family Alcaligenaceae . Both strains showed 16S rRNA gene sequence similarity of 100 % to each other. The closest relatives of the isolates were Pigmentiphaga daeguensis (97.08 % sequence similarity), Pigmentiphaga kullae (97.01 %) and Pigmentiphaga litoralis (96.73 %). On the basis of data from phenotypic, chemotaxonomic and phylogenetic analyses, SAP-32T (=KCTC 52619T=DSM 104039T) and SAP-36 (=KCTC 52620=DSM 104072) represent members of a novel species of the genus Pigmentiphaga , for which the name Pigmentiphaga aceris sp. nov. is proposed.", }