1887

Abstract

A Gram-stain-negative, non-motile, aerobic, non-spore-forming, spherical bacterium (strain MIMtkB18) was isolated from desert soil collected from part of a Mongolian Plateau, territory of Inner Mongolia, PR China. Cell growth could be observed at 20–45 °C (optimum at 40 °C), at a pH of 6–9 (optimum at pH 8.6) and in the presence of 0–1 % (w/v) NaCl (optimum 0 %). The genomic DNA G+C content was 69.6 mol%. 16S rRNA gene sequence analysis showed that strain MIMtkB18 was most closely related to Methylobrevis pamukkalensis PK2 (94.1 %), species of the genus Pleomorphomonas (93.4–94.0 %), and Hartmannibacter diazotrophicus E19 (93.9 %). The sole respiratory quinone was Q-10. The major fatty acids (>5 %) were C18 : 0 (5.7 %) and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) (81.6 %). Polar lipids were mainly composed of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine and unidentified phospholipids. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, it is concluded that strain MIMtkB18 represents a novel genus and species, for which the name Mongoliimonas terrestris sp. nov. is proposed. The type strain is MIMtkB18 (=KCTC 42635=MCCC 1K00571).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.002067
2017-08-18
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/8/3010.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.002067&mimeType=html&fmt=ahah

References

  1. Du H, Jiao N, Hu Y, Zeng Y. Diversity and distribution of pigmented heterotrophic bacteria in marine environments. FEMS Microbiol Ecol 2006;57:92–105 [CrossRef][PubMed]
    [Google Scholar]
  2. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017;67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  3. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol 1990;215:403–410 [CrossRef][PubMed]
    [Google Scholar]
  4. Kimura M, Takahata N. Selective constraint in protein polymorphism: study of the effectively neutral mutation model by using an improved pseudosampling method. Proc Natl Acad Sci USA 1983;80:1048–1052 [CrossRef][PubMed]
    [Google Scholar]
  5. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  6. Poroshina MN, Trotsenko YA, Doronina NV. Methylobrevis pamukkalensis gen. nov., sp. nov., a halotolerant restricted facultative methylotroph isolated from saline water. Int J Syst Evol Microbiol 2015;65:1321–1327 [CrossRef][PubMed]
    [Google Scholar]
  7. Xie CH, Yokota A. Pleomorphomonas oryzae gen. nov., sp. nov., a nitrogen-fixing bacterium isolated from paddy soil of Oryza sativa. Int J Syst Evol Microbiol 2005;55:1233–1237 [CrossRef][PubMed]
    [Google Scholar]
  8. Im WT, Kim SH, Kim MK, Ten LN, Lee ST. Pleomorphomonas koreensis sp. nov., a nitrogen-fixing species in the order Rhizobiales. Int J Syst Evol Microbiol 2006;56:1663–1666 [CrossRef][PubMed]
    [Google Scholar]
  9. Madhaiyan M, Jin TY, Roy JJ, Kim SJ, Weon HY et al. Pleomorphomonas diazotrophica sp. nov., an endophytic N-fixing bacterium isolated from root tissue of Jatropha curcas L. Int J Syst Evol Microbiol 2013;63:2477–2483 [CrossRef][PubMed]
    [Google Scholar]
  10. Suarez C, Ratering S, Geissler-Plaum R, Schnell S. Hartmannibacter diazotrophicus gen. nov., sp. nov., a phosphate-solubilizing and nitrogen-fixing alphaproteobacterium isolated from the rhizosphere of a natural salt-meadow plant. Int J Syst Evol Microbiol 2014;64:3160–3167 [CrossRef][PubMed]
    [Google Scholar]
  11. Lanyi B. Classical and rapid identification methods for medically important Bacteria. Method Microbiol 1988;19:1–67[CrossRef]
    [Google Scholar]
  12. Skerman V. A Guide to the Identification of the Genera of Bacteria, 2nd ed. Baltimore: Williams & Wilkins; 1967
    [Google Scholar]
  13. Dong X, Cai M. Determinative Manual for Routine Bacteriology Beijing: Scientific Press (English translation); 2001
    [Google Scholar]
  14. Tindall BJ, Sikorski J, Smibert RA, Krieg NR. Phenotypic characterization and the principles of comparative systematics. In Reddy CA, Beveridge TJ, Breznak JA, Marzluf G, Schmidt TM. et al. (editors) Methods for General and Molecular Microbiology, 3rd ed. Washington, DC: American Society for Microbiology; 2007; pp.330–393
    [Google Scholar]
  15. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984;2:233–241 [CrossRef]
    [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  17. Du Y, Yuan B, Zeng Y, Meng J, Li H et al. Draft genome sequence of the cellulolytic bacterium Clavibacter sp. CF11, a strain producing cold-active cellulase. Genome Announc 2015;3:e01304-14e01314 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.002067
Loading
/content/journal/ijsem/10.1099/ijsem.0.002067
Loading

Data & Media loading...

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error