1887

Abstract

In previous studies, two hydrocarbon-degrading sulfate-reducing bacteria, strains PP31 and PL12, were obtained from oil-polluted marine sediments of Shuaiba, Kuwait. They had been reported as organisms capable of anaerobic degradation of p-xylene and n-alkanes, respectively. The 16S rRNA gene sequence of strain PP31 showed 98.8 % sequence similarities to that of Desulfosarcina variabilis ‘Montpellier’. Strains PL12 had 97.8 % of sequence similarity to Desulfosarcina ovata oXys1. They both have been partially characterized, but not been validly published as new species of the genus Desulfosarcina . In this study, additional characterizations of these strains were made to describe them as two new species of the genus Desulfosarcina . Major cellular fatty acids of strain PP31 were C15 : 0 (25.9 %) and anteiso-C15 : 0 (22.3 %), whereas those of strain PL12 were C15 : 0 (21.3 %), C16 : 0 (17.8 %) and anteiso-15 : 0 (11.6 %). The phylogenetic tree based on 16S rRNA gene revealed that these isolates should not be classified as any of the known species in the genus Desulfosarcina . On the basis of phenotypic and phylogenetic analyses, these two sulfate reducers are proposed to form two novel species of the genus Desulfosarcina : Desulfosarcina widdelii sp. nov. (PP31=JCM 31729=DSM 103921) and Desulfosarcina alkanivorans sp. nov. (PL12=JCM 31728=DSM 103901). In addition, emended description of the genus Desulfosarcina is presented in this study.

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2017-08-18
2019-12-11
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