1887

Abstract

One strain, designated 63MJ-1, was isolated from fresh faeces of broad-winged katydids collected in Jinan-gun, Jeollabuk-do, the Republic of Korea. The organism stained Gram-positive and was an aerobic, non-flagellated and short-rod-shaped bacterium. The organism grew in the range of 4–35 °C (optimum, 28–30 °C) and pH 6.0–9.0 (optimum, pH 7.0), and in the presence of 5 % NaCl (w/v), but not in media containing 7 % NaCl. According to the 16S rRNA gene sequence analysis, strain 63MJ-1 showed the highest sequence similarities with P4-7 (95.9 %), 2Q3S-4-2 (95.8 %) and DSM 44233 (95.7 %). Phylogenetic trees also indicated that strain 63MJ-1 formed one robust cluster with members of the genus. The predominant quinone of strain 63MJ-1 was MK-8(H). Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified aminophospholipid and two unidentified lipids. The major fatty acids were C, anteiso-C and iso-C. The peptidoglycan type was A1 with -diaminopimelic acid as the diagnostic amino acid. The DNA G+C content was 64.6 mol%. Based on the phylogenetic, physiological and chemotaxonomic data, it was demonstrated that strain 63MJ-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 63MJ-1 (=KACC 18662=NBRC 111844).

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2017-08-01
2024-12-06
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References

  1. Yoshimi Y, Hiraishi A, Nakamura K. Isolation and characterization of Microsphaera multipartita gen. nov., sp. nov., a polysaccharide-accumulating Gram-positive bacterium from activated sludge. Int J Syst Bacteriol 1996; 46:519–525 [View Article]
    [Google Scholar]
  2. Tao TS, Yue YY, Chen WX, Chen WF. Proposal of Nakamurella gen. nov. as a substitute for the bacterial genus Microsphaera Yoshimi et al. 1996 and Nakamurellaceae fam. nov. as a substitute for the illegitimate bacterial family Microsphaeraceae Rainey et al. 1997. Int J Syst Evol Microbiol 2004; 54:999–1000 [View Article][PubMed]
    [Google Scholar]
  3. Tuo L, Li FN, Pan Z, Lou I, Guo M et al. Nakamurella endophytica sp. nov., a novel endophytic actinobacterium isolated from the bark of Kandelia candel. Int J Syst Evol Microbiol 2016; 66:1577–1582 [View Article][PubMed]
    [Google Scholar]
  4. Kim KK, Lee KC, Lee JS. Nakamurella panacisegetis sp. nov. and proposal for reclassification of Humicoccus flavidus Yoon et al., 2007 and Saxeibacter lacteus Lee et al., 2008 as Nakamurella flavida comb. nov. and Nakamurella lactea comb. nov. Syst Appl Microbiol 2012; 35:291–296 [View Article][PubMed]
    [Google Scholar]
  5. França L, Albuquerque L, Zhang DC, Nouioui I, Klenk HP et al. Nakamurella silvestris sp. nov., an actinobacterium isolated from alpine forest soil. Int J Syst Evol Microbiol 2016; 66:5460–5464 [View Article][PubMed]
    [Google Scholar]
  6. Lee SD, Park SK, Yun YW, Lee DW. Saxeibacter lacteus gen. nov., sp. nov., an actinobacterium isolated from rock. Int J Syst Evol Microbiol 2008; 58:906–909 [View Article][PubMed]
    [Google Scholar]
  7. Yoon JH, Kang SJ, Jung SY, Oh TK. Humicoccus flavidus gen. nov., sp. nov., isolated from soil. Int J Syst Evol Microbiol 2007; 57:56–59 [View Article][PubMed]
    [Google Scholar]
  8. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
    [Google Scholar]
  9. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  10. Ludwig W, Strunk O, Westram R, Richter L, Meier H et al. ARB: a software environment for sequence data. Nucleic Acids Res 2004; 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  11. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  12. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 1993; 10:512–526[PubMed]
    [Google Scholar]
  13. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  14. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  15. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  16. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101 Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  17. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  18. Gonzalez JM, Saiz-Jimenez C. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 2002; 4:770–773 [View Article][PubMed]
    [Google Scholar]
  19. Hamada M, Yamamura H, Komukai C, Tamura T, Suzuki K et al. Luteimicrobium album sp. nov., a novel actinobacterium isolated from a lichen collected in Japan, and emended description of the genus Luteimicrobium. J Antibiot 2012; 65:427–431 [View Article][PubMed]
    [Google Scholar]
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