RT Journal Article SR Electronic(1) A1 Wang, Guanghua A1 Yu, Kefu A1 Wang, Yinghui A1 Su, Hongfei A1 Wu, Hualian A1 Li, Tao A1 Liang, Jiayuan A1 Huang, Wen A1 Xiang, WenzhouYR 2017 T1 Pelagibacterium lentulum sp. nov., a marine bacterium from the culture broth of Picochlorum sp. 122 JF International Journal of Systematic and Evolutionary Microbiology, VO 67 IS 9 SP 3182 OP 3185 DO https://doi.org/10.1099/ijsem.0.002054 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, motile, non-spore-forming, rod-shaped bacterium, designated strain B2T, was isolated from the culture broth of a marine microalga, Picochlorum sp. 122. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain B2T forms a robust cluster with members of the genus Pelagibacterium , and shares the highest sequence similarity of 96.80 % with Pelagibacterium halotolerans CGMCC 1.7692T. Optimal growth of strain B2T was observed at 33 °C, at pH 8 and in the presence of 1 % (w/v) NaCl. The predominant ubiquinone of strain B2T was Q-10, and the G+C content of the genomic DNA was 58.6 mol%. The major fatty acid profile comprised C18 : 1ω7c /ω6c, C19 : 0 cyclo ω8c and C16 : 0. The major polar lipids of strain B2T were diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and seven unidentified lipids. Comprehensive analyses based on a polyphasic characterization of strain B2T indicated that it represents a novel species of the genus Pelagibacterium , for which the name Pelagibacterium lentulum sp. nov. is proposed. The type strain is B2T (=MCCC 1K03218T=CGMCC 1.15896T=KCTC 52551T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.002054