sp. nov., a novel member of the genus isolated from a saltern Free

Abstract

A moderately halophilic bacterium, designated strain SP30, was isolated from a solar saltern located in Santa Pola, Alicante, on the East coast of Spain. It was a Gram-stain-negative, strictly aerobic bacterium, able to grow in 7.5–25 % (w/v) NaCl and optimally in 12.5 % (w/v) NaCl. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that the novel isolate is a member of the genus , with the most closely related species being SSL50 (99.9 % sequence similarity) and UAH-SP71 (99.4 % sequence similarity). The 16S rRNA gene sequence similarity with the type species M19-40 was 96.6 %. The DNA–DNA relatedness value between strain SP30 and SSL50 and UAH-SP71 was 40 and 55 %, respectively; these values are lower than the 70 % threshold accepted for species delineation. The major fatty acids were C Cω7, C cyclo ω8 and C. Similarly to other species of the genus , strain SP30was observed as curved rods and spiral cells. Metabolic versatility was reduced to the utilization of a few organic compounds as the sole carbon and energy sources, as with other members of However, it differed in terms of colony pigmentation (brownish-yellow instead of pink) and in having a higher growth rate. Based on these data and on the phenotypic, genotypic and chemotaxonomic characterization, we propose the classification of strain SP30 as a novel species within the genus , with the name sp. nov. The type strain is SP30 (=CECT 9238=LMG 30005).

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2017-08-01
2024-03-28
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