1887

Abstract

A moderately halophilic bacterium, designated strain SP30, was isolated from a solar saltern located in Santa Pola, Alicante, on the East coast of Spain. It was a Gram-stain-negative, strictly aerobic bacterium, able to grow in 7.5–25 % (w/v) NaCl and optimally in 12.5 % (w/v) NaCl. Phylogenetic analyses, based on 16S rRNA gene sequences, showed that the novel isolate is a member of the genus , with the most closely related species being SSL50 (99.9 % sequence similarity) and UAH-SP71 (99.4 % sequence similarity). The 16S rRNA gene sequence similarity with the type species M19-40 was 96.6 %. The DNA–DNA relatedness value between strain SP30 and SSL50 and UAH-SP71 was 40 and 55 %, respectively; these values are lower than the 70 % threshold accepted for species delineation. The major fatty acids were C Cω7, C cyclo ω8 and C. Similarly to other species of the genus , strain SP30was observed as curved rods and spiral cells. Metabolic versatility was reduced to the utilization of a few organic compounds as the sole carbon and energy sources, as with other members of However, it differed in terms of colony pigmentation (brownish-yellow instead of pink) and in having a higher growth rate. Based on these data and on the phenotypic, genotypic and chemotaxonomic characterization, we propose the classification of strain SP30 as a novel species within the genus , with the name sp. nov. The type strain is SP30 (=CECT 9238=LMG 30005).

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2017-08-01
2024-12-03
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References

  1. Ventosa A. Unusual micro-organisms from unusual habitats: hypersaline environments. In Logan NA, Lappin-Scott HM, Oyston PCF. (editors) Prokaryotic Diversity: Mechanism and Significance Cambridge: Cambridge University Press; 2006 pp. 223–253 [CrossRef]
    [Google Scholar]
  2. Oren A. Ecology of halophiles. In Horikoshi K, Antranikian G, Bull A, Robb F, Stetter K et al. (editors) Extremophiles Handbook Heidelberg: Springer; 2011 pp. 344–361
    [Google Scholar]
  3. Ventosa A, de la Haba RR, Sánchez-Porro C, Papke RT. Microbial diversity of hypersaline environments: a metagenomic approach. Curr Opin Microbiol 2015; 25:80–87 [View Article][PubMed]
    [Google Scholar]
  4. Ghai R, Pašić L, Fernández AB, Martin-Cuadrado AB, Mizuno CM et al. New abundant microbial groups in aquatic hypersaline environments. Sci Rep 2011; 1:1–10 [View Article][PubMed]
    [Google Scholar]
  5. Fernández AB, Ghai R, Martin-Cuadrado AB, Sánchez-Porro C, Rodriguez-Valera F et al. Prokaryotic taxonomic and metabolic diversity of an intermediate salinity hypersaline habitat assessed by metagenomics. FEMS Microbiol Ecol 2014; 88:623–635 [View Article][PubMed]
    [Google Scholar]
  6. Ventosa A, Fernández AB, León MJ, Sánchez-Porro C, Rodriguez-Valera F. The Santa Pola saltern as a model for studying the microbiota of hypersaline environments. Extremophiles 2014; 18:811–824 [View Article][PubMed]
    [Google Scholar]
  7. Fernández AB, Vera-Gargallo B, Sánchez-Porro C, Ghai R, Papke RT et al. Comparison of prokaryotic community structure from mediterranean and atlantic saltern concentrator ponds by a metagenomic approach. Front Microbiol 2014; 5:196 [View Article][PubMed]
    [Google Scholar]
  8. León MJ, Fernández AB, Ghai R, Sánchez-Porro C, Rodriguez-Valera F et al. From metagenomics to pure culture: isolation and characterization of the moderately halophilic bacterium Spiribacter salinus gen. nov., sp. nov. Appl Environ Microbiol 2014; 80:3850–3857 [View Article][PubMed]
    [Google Scholar]
  9. León MJ, Rodríguez-Olmos A, Sánchez-Porro C, López-Pérez M, Rodríguez-Valera F et al. Spiribacter curvatus sp. nov., a new moderately halophilic bacterium from Santa Pola saltern in Spain. Int J Syst Evol Microbiol 2015; 65:4638–4643 [CrossRef]
    [Google Scholar]
  10. León MJ, Vera-Gargallo B, Sánchez-Porro C, Ventosa A. Spiribacter roseus sp. nov., a moderately halophilic species of the genus Spiribacter from salterns. Int J Syst Evol Microbiol 2016; 66:4218–4224 [View Article][PubMed]
    [Google Scholar]
  11. López-Pérez M, Ghai R, Leon MJ, Rodríguez-Olmos Á, Copa-Patiño JL et al. Genomes of "Spiribacter", a streamlined, successful halophilic bacterium. BMC Genomics 2013; 14:787 [View Article][PubMed]
    [Google Scholar]
  12. Dillon JG, Carlin M, Gutierrez A, Nguyen V, Mclain N. Patterns of microbial diversity along a salinity gradient in the Guerrero Negro solar saltern, Baja CA Sur, Mexico. Front Microbiol 2013; 4:399 [View Article][PubMed]
    [Google Scholar]
  13. Quiroz M, Triadó-Margarit X, Casamayor EO, Gajardo G. Comparison of Artemia-bacteria associations in brines, laboratory cultures and the gut environment: a study based on chilean hypersaline environments. Extremophiles 2015; 19:135–147 [View Article][PubMed]
    [Google Scholar]
  14. Zhang J, Ma G, Deng Y, Dong J, van Stappen G et al. Bacterial diversity in Bohai Bay solar saltworks, China. Curr Microbiol 2016; 72:55–63 [View Article][PubMed]
    [Google Scholar]
  15. Sánchez-Porro C, de la Haba RR, Soto-Ramírez N, Márquez MC, Montalvo-Rodríguez R et al. Description of Kushneria aurantia gen. nov., sp. nov., a novel member of the family Halomonadaceae, and a proposal for reclassification of Halomonas marisflavi as Kushneria marisflavi comb. nov., of Halomonas indalinina as Kushneria indalinina comb. nov. and of Halomonas avicenniae as Kushneria avicenniae comb. nov. Int J Syst Evol Microbiol 2009; 59:397–405 [View Article][PubMed]
    [Google Scholar]
  16. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956; 178:703 [View Article][PubMed]
    [Google Scholar]
  17. Cowan ST, Steel KJ. Manual for the Identification of Medical Bacteria London: Cambridge University Press; 1965
    [Google Scholar]
  18. Ventosa A, Quesada E, Rodriguez-Valera F, Ruiz-Berraquero F, Ramos-Cormenzana A. Numerical taxonomy of moderately halophilic Gram-negative rods. Microbiology 1982; 128:1959–1968 [View Article]
    [Google Scholar]
  19. Koser SA. Utilization of the salts of organic acids by the colon-aerogenes group. J Bacteriol 1923; 8:493–520[PubMed]
    [Google Scholar]
  20. Marmur J. A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 1961; 3:208–IN1 [View Article]
    [Google Scholar]
  21. Márquez MC, Carrasco IJ, Xue Y, Ma Y, Cowan DA et al. Aquisalibacillus elongatus gen. nov., sp. nov., a moderately halophilic bacterium of the family Bacillaceae isolated from a saline lake. Int J Syst Evol Microbiol 2008; 58:1922–1926 [View Article][PubMed]
    [Google Scholar]
  22. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  23. Ludwig W, Strunk O, Westram R, Richter L, Meier H et al. ARB: a software environment for sequence data. Nucleic Acids Res 2004; 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  24. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 1971; 20:406–416 [View Article]
    [Google Scholar]
  25. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  26. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  27. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  28. Marmur J, Doty P. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 1962; 5:109–118 [View Article][PubMed]
    [Google Scholar]
  29. Owen RJ, Hill LR. The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Skinner FA, Lovelock DW. (editors) Identification Methods for Microbiologists, 2nd ed. London: Academic Press; 1979 pp. 217–296
    [Google Scholar]
  30. Imhoff JF. Reassignment of the genus Ectothiorhodospira Pelsh 1936 to a new family, Ectothiorhodospiraceae fam. nov., and emended description of the Chromatiaceae Bavendamm 1924. Int J Syst Bacteriol 1984; 34:338–339 [View Article]
    [Google Scholar]
  31. Johnson JL. Similarity analysis of DNAs. In Gerhardh P, Murray RGE, Wood WA, Krieg NR. (editors) Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp. 655–681
    [Google Scholar]
  32. de Ley J, Tijtgat R. Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 1970; 36:461–474 [View Article][PubMed]
    [Google Scholar]
  33. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [View Article]
    [Google Scholar]
  34. Stackebrandt E, Frederiksen W, Garrity GM, Grimont PA, Kämpfer P et al. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 2002; 52:1043–1047 [View Article][PubMed]
    [Google Scholar]
  35. MIDI Sherlock Microbial Identification System Operating Manual, Version 6.1 Newyark, DE: MIDI Inc; 2008
    [Google Scholar]
  36. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  37. Peña A, Valens M, Santos F, Buczolits S, Antón J et al. Intraspecific comparative analysis of the species Salinibacter ruber. Extremophiles 2005; 9:151–161 [View Article][PubMed]
    [Google Scholar]
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