1887

Abstract

A novel endophytic bacterium, strain J11-6, was isolated from rice stems. Its taxonomic position was investigated using a polyphasic approach. The novel strain was Gram-staining-negative, facultatively anaerobic, motile and rod-shaped. Although the results of phylogenetic analysis based on 16S rRNA gene sequences indicated that J11-6 represented a member of the genus Rahnella , multilocus sequence analysis (MLSA) on the basis of concatenated partial atpD, gyrB, rpoB and infB gene sequences showed a clear distinction of J11-6 from the type strains of species of the genus Rahnella but indicated that it lay within the clade of the genus Serratia . The phylogenetically closest species were Serratia fonticola and Serratia aquatilis on the basis of the results of the MLSA phylogenetic analysis. The predominant cellular fatty acids were C16 : 1ω7c (38.7 %) and C16 : 0 (25.0 %). The DNA G+C content was 53.2 mol%. The DNA–DNA relatedness was 17.4 % between J11-6 and Rahnella aquatilis CIP 78.65, and 29.2 % between J11-6 and S. fonticola LMG 7882 which indicates that this strain represents a novel species of the genus Serratia . Characterization by genotypic and phenotypic analysis indicated that J11-6 (=ACCC 19934=KCTC 52529) represents a novel species of the genus Serratia , for which the name Serratia oryzae sp. nov. is proposed.

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2017-08-30
2019-10-24
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