1887

Abstract

A novel endophytic bacterium, strain J11-6, was isolated from rice stems. Its taxonomic position was investigated using a polyphasic approach. The novel strain was Gram-staining-negative, facultatively anaerobic, motile and rod-shaped. Although the results of phylogenetic analysis based on 16S rRNA gene sequences indicated that J11-6 represented a member of the genus , multilocus sequence analysis (MLSA) on the basis of concatenated partial and gene sequences showed a clear distinction of J11-6 from the type strains of species of the genus but indicated that it lay within the clade of the genus . The phylogenetically closest species were and on the basis of the results of the MLSA phylogenetic analysis. The predominant cellular fatty acids were Cω7 (38.7 %) and C (25.0 %). The DNA G+C content was 53.2 mol%. The DNA–DNA relatedness was 17.4 % between J11-6 and CIP 78.65, and 29.2 % between J11-6 and LMG 7882 which indicates that this strain represents a novel species of the genus . Characterization by genotypic and phenotypic analysis indicated that J11-6 (=ACCC 19934=KCTC 52529) represents a novel species of the genus , for which the name sp. nov. is proposed.

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2017-08-01
2022-06-27
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