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Volume 67, Issue 8

sp. nov., an endophytic actinomycete isolated from the stem of Free

Abstract

A novel endophytic actinomycete, designated strain SC1-1, was isolated from sterilized stem tissue from collected in Udon Thani province, Thailand. The isolate formed short chains of spores on aerial mycelium and presented -diaminopimelic acid in the cell wall peptidoglycan. Glucose, madurose, mannose, rhamnose and ribose were observed as sugars in the cells. The cell membrane phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxy-phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and ninhydrin-positive phosphoglycolipids. The major menaquinones were MK-9(H) and MK-9(H). The main cellular fatty acids were iso-C, 10-methyl C and Cω6. A high G+C content (70.7 mol%) was present in the genomic DNA. The taxonomic position based on the 16S rRNA gene sequence analysis revealed that strain SC1-1 belonged to the genus and shared the highest 16S rRNA gene sequence similarity value with DSM 44320 (98.82 %), followed by IFO 14745 (98.58 %), A4036 (98.43 %), YIM 65601 (98.36 %), 1SM4-01 (98.34 %), IFO 13155 (98.29 %), IFO 14685 (98.23 %) and IFO 14525 (98.23 %). On the basis of the DNA–DNA hybridization relatedness and including the physiological and biochemical characteristics, strain SC1-1 should be judged as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain SC1-1 (=BCC 66361=NBRC 110006).

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Keyword(s): endophytic actinomycetes , Nonomuraea and Stahlianthus campanulatus
© 2017 IUMS
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2017-08-01
2024-04-19
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Nonomuraea stahlianthi sp. nov., an endophytic actinomycete isolated from the stem of Stahlianthus campanulatus
Int J Syst Evol Microbiol 67, 2879 (2017); https://doi.org/10.1099/ijsem.0.002045
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