1887

Abstract

A novel endophytic actinomycete, designated strain SC1-1, was isolated from sterilized stem tissue from Stahlianthus campanulatus collected in Udon Thani province, Thailand. The isolate formed short chains of spores on aerial mycelium and presented meso-diaminopimelic acid in the cell wall peptidoglycan. Glucose, madurose, mannose, rhamnose and ribose were observed as sugars in the cells. The cell membrane phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxy-phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and ninhydrin-positive phosphoglycolipids. The major menaquinones were MK-9(H4) and MK-9(H2). The main cellular fatty acids were iso-C16:0, 10-methyl C17 : 0 and C17 : 1ω6c. A high G+C content (70.7 mol%) was present in the genomic DNA. The taxonomic position based on the 16S rRNA gene sequence analysis revealed that strain SC1-1 belonged to the genus Nonomuraea and shared the highest 16S rRNA gene sequence similarity value with Nonomuraea dietziae DSM 44320 (98.82 %), followed by Nonomuraea africana IFO 14745 (98.58 %), Nonomuraea jabiensis A4036 (98.43 %), Nonomuraea endophytica YIM 65601 (98.36 %), Nonomuraea purpurea 1SM4-01 (98.34 %), Nonomuraea angiospora IFO 13155 (98.29 %), Nonomuraea roseola IFO 14685 (98.23 %) and Nonomuraea recticatena IFO 14525 (98.23 %). On the basis of the DNA–DNA hybridization relatedness and including the physiological and biochemical characteristics, strain SC1-1 should be judged as a novel species of the genus Nonomuraea , for which the name Nonomuraea stahlianthi sp. nov. is proposed. The type strain is strain SC1-1 (=BCC 66361=NBRC 110006).

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2017-08-30
2019-10-16
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