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Abstract

A Gram-stain-positive, rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-27215, was isolated from grass soil collected from Wudalianchi in the Heilongjiang Province of China. Growth was observed at 10–60 °C (optimum 30 °C), in 0 and 3.0 % NaCl (optimum 0 %) and at pH 5.0–10.0 (optimum 7.0), respectively. The cell-wall peptidoglycan contained -diaminopimelic acid and the isoprenoid quinone was MK7. The main fatty acids were iso-C, anteiso-C, anteiso-C, and iso-C. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidyl ethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-27215 to the genus . Strain FJAT-27215 showed high sequence similarities to SGD-V-25 (98.6 %), NBRC 15713 (98.6 %), SD111 (96.9 %) and SgZ-8 (96.5 %). The average nucleotide identity values between strain FJAT-27215 and the type strains of closely related species were much lower than the 96 % threshold value for delineation of genomic prokaryotic species. The DNA–DNA hybridization values between strain FJAT-27215 and the most closely related strain SGD-V-25 showed a similarity of 22.4 % and lower than 70 %, indicating that they belong to different taxa. The phenotypic characters and taxono-genomics study revealed that strain FJAT-27215 represents a novel species, for which the name sp. nov. is proposed. The type strain is FJAT-27215 (=CCTCC AB 2015266=DSM 100757).

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2017-08-01
2021-07-25
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